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Nepravishta, Ridvan, Monaco, Serena, Distefano, Marco, Rizzo, Roberto, Cescutti, Paola and Angulo, Jesus 
ORCID: https://orcid.org/0000-0001-7250-5639
(2021)
Multifrequency STD NMR unveils the interactions of antibiotics with Burkholderia multivorans biofilm exopolysaccharide.
Frontiers in Molecular Biosciences, 8.
ISSN 2296-889X
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Abstract
Biofilms confine bacterial cells within self-produced matrices, offering advantages such as protection from antibiotics and entrapment of nutrients. Polysaccharides are major components in these macromolecular assemblies, and their interactions with other chemicals are of high relevance for the benefits provided by the biofilm 3D molecular matrix. NMR is a powerful technique for the study and characterization of the interactions between molecules of biological relevance. In this study, we have applied multifrequency saturation transfer difference (STD) NMR and DOSY NMR approaches to elucidate the interactions between the exopolysaccharide produced by Burkholderia multivorans C1576 (EpolC1576) and the antibiotics kanamycin and ceftadizime. The NMR strategies presented here allowed for an extensive characterization at an atomic level of the mechanisms behind the implication of the EpolC1576 in the recalcitrance phenomena, which is the ability of bacteria in biofilms to survive in the presence of antibiotics. Our results suggest an active role for EpolC1576 in the recalcitrance mechanisms toward kanamycin and ceftadizime, though through two different mechanisms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | burkholderia multivorans,std nmr,biofilms,exopolysaccharides,multifrequency std nmr,biochemistry,biochemistry, genetics and molecular biology (miscellaneous),molecular biology ,/dk/atira/pure/subjectarea/asjc/1300/1303 |
| Faculty \ School: | Faculty of Science > School of Pharmacy |
| UEA Research Groups: | Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 15 Oct 2021 01:13 |
| Last Modified: | 23 Oct 2022 03:08 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/81720 |
| DOI: | 10.3389/fmolb.2021.727980 |
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