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Olaya-Abril, Alfonso, Hidalgo-Carrillo, Jesús, Luque-Almagro, Víctor M., Fuentes-Almagro, Carlos, Urbano, Francisco J., Moreno-Vivián, Conrado, Richardson, David J. 
ORCID: https://orcid.org/0000-0002-6847-1832 and Roldán, María Dolores
(2021)
Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222.
Scientific Reports, 11.
ISSN 2045-2322
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Abstract
Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N 2O/N 2 ratios compared to alkaline soils. In this work, impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with nitrate as sole energy and nitrogen source under anaerobic conditions at pH ranging from 6.5 to 7.5. Quantitative proteomic analysis revealed that the highest difference in protein representation was observed when the proteome at pH 6.5 was compared to the reference proteome at pH 7.2. However, this difference in the extracellular pH was not enough to produce modification of intracellular pH, which was maintained at 6.5 ± 0.1. The biosynthetic pathways of several cofactors relevant for denitrification and nitrogen assimilation like cobalamin, riboflavin, molybdopterin and nicotinamide were negatively affected at pH 6.5. In addition, peptide representation of reductases involved in nitrate assimilation and denitrification were reduced at pH 6.5. Data highlight the strong negative impact of pH on NosZ synthesis and intracellular copper content, thus impairing active NosZ assembly and, in turn, leading to elevated nitrous oxide emissions.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Organisms and the Environment |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 02 Sep 2021 00:18 |
| Last Modified: | 31 Jan 2024 03:09 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/81277 |
| DOI: | 10.1038/s41598-021-96559-2 |
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