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He, Can-Xia, Buongiorno, Luigina, Wang, Wei, Tang, Jonathan C. Y. 
ORCID: https://orcid.org/0000-0001-6305-6333, Miceli, Natalizia, Taviano, Maria Fernanda, Shan, Yujuan and Bao, Yongping ORCID: https://orcid.org/0000-0002-6425-0370
(2021)
The inhibitory effect of sulforaphane on bladder cancer cell depends on GSH depletion-induced by Nrf2 translocation.
Molecules, 26 (16).
ISSN 1420-3049
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Abstract
Sulforaphane (SFN), an isothiocyanate (ITCs) derived from glucosinolate that is found in cruciferous vegetables, has been reported to exert a promising anticancer effect in a substan-tial amount of scientific research. However, epidemical studies showed inconsistencies between cruciferous vegetable intake and bladder cancer risk. In this study, human bladder cancer T24 cells were used as in vitro model for revealing the inhibitory effect and its potential mechanism of SFN on cell growth. Here, a low dose of SFN (2.5 µM) was shown to promote cell prolifera-tion (5.18–11.84%) and migration in T24 cells, whilst high doses of SFN (>10 µM) inhibited cell growth significantly. The induction effect of SFN on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression at both low (2.5 µM) and high dose (10 µM) was characterized by a bell-shaped curve. Nrf2 and glutathione (GSH) might be the underlying mechanism in the effect of SFN on T24 cell growth since Nrf2 siRNA and GSH-depleting agent L-Buthionine-sulfoximine abolished the effect of SFN on cell proliferation. In summary, the inhibitory effect of SFN on bladder can-cer cell growth and migration is highly dependent on Nrf2-mediated GSH depletion and fol-lowing production. These findings suggested that a higher dose of SFN is required for the pre-vention and treatment of bladder cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bladder cancer,chemoprevention,glutathione,nrf2,sulforaphane,analytical chemistry,chemistry (miscellaneous),molecular medicine,pharmaceutical science,drug discovery,physical and theoretical chemistry,organic chemistry,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1600/1602 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Medicine and Health Sciences > Research Groups > Cancer Studies Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 17 Aug 2021 00:18 |
| Last Modified: | 13 Nov 2023 17:50 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/81118 |
| DOI: | 10.3390/molecules26164919 |
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