Bespoke biomolecular wires for transmembrane electron transfer: Spontaneous assembly of a functionalized multiheme electron conduit

Piper, Samuel, Edwards, Marcus, van Wonderen, Jessica, Casadevall, Carla, Martel, Anne, Jeuken, Lars J. C., Reisner, Erwin, Clarke, Tom ORCID: https://orcid.org/0000-0002-6234-1914 and Butt, Julea ORCID: https://orcid.org/0000-0002-9624-5226 (2021) Bespoke biomolecular wires for transmembrane electron transfer: Spontaneous assembly of a functionalized multiheme electron conduit. Frontiers in Microbiology, 12. ISSN 1664-302X

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Abstract

Shewanella oneidensis exchanges electrons between cellular metabolism and external redox partners in a process that attracts much attention for production of green electricity (microbial fuel cells) and chemicals (microbial electrosynthesis). A critical component of this pathway is the outer membrane spanning MTR complex, a biomolecular wire formed of the MtrA, MtrB, and MtrC proteins. MtrA and MtrC are decaheme cytochromes that form a chain of close-packed hemes to define an electron transfer pathway of 185 Å. MtrA is wrapped inside MtrB for solubility across the outer membrane lipid bilayer; MtrC sits outside the cell for electron exchange with external redox partners. Here, we demonstrate tight and spontaneous in vitro association of MtrAB with separately purified MtrC. The resulting complex is comparable with the MTR complex naturally assembled by Shewanella in terms of both its structure and rates of electron transfer across a lipid bilayer. Our findings reveal the potential for building bespoke electron conduits where MtrAB combines with chemically modified MtrC, in this case, labeled with a Ru-dye that enables light-triggered electron injection into the MtrC heme chain.

Item Type:	Article
Uncontrolled Keywords:	sans,shewanella,biomolecular wire,cytochrome,electron transfer,microbial electrosynthesis,microbial fuel cell,photosensitizer,microbiology,microbiology (medical),2* ,/dk/atira/pure/subjectarea/asjc/2400/2404
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry Faculty of Science > School of Natural Sciences
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	14 Jul 2021 00:29
Last Modified:	20 Apr 2023 21:36
URI:	https://ueaeprints.uea.ac.uk/id/eprint/80554
DOI:	10.3389/fmicb.2021.714508

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