A novel allele of the Arabidopsis thaliana MACPF protein CAD1 results in deregulated immune signaling

Holmes, Danalyn R., Bredow, Melissa, Thor, Kathrin, Pascetta, Sydney A., Sementchoukova, Irina, Siegel, Kristen R., Zipfel, Cyril and Monaghan, Jacqueline (2021) A novel allele of the Arabidopsis thaliana MACPF protein CAD1 results in deregulated immune signaling. Genetics, 217 (4). ISSN 1943-2631

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Abstract

Immune recognition in plants is governed by two major classes of receptors: pattern recognition receptors (PRRs) and nucleotide-binding leucine-rich repeat receptors (NLRs). Located at the cell surface, PRRs bind extracellular ligands originating from microbes (indicative of 'non-self') or damaged plant cells (indicative of 'infected-self'), and trigger signaling cascades to protect against infection. Located intracellularly, NLRs sense pathogen-induced physiological changes and trigger localized cell death and systemic resistance. Immune responses are under tight regulation in order to maintain homeostasis and promote plant health. In a forward-genetic screen to identify regulators of PRR-mediated immune signaling, we identified a novel allele of the membrane-attack complex and perforin (MACPF)-motif containing protein CONSTITUTIVE ACTIVE DEFENSE 1 (CAD1) resulting from a missense mutation in a conserved N-terminal cysteine. We show that cad1-5 mutants display deregulated immune signaling and symptoms of autoimmunity dependent on the lipase-like protein ENHANCED DISEASE SUSCEPTIBILITY1 (EDS1), suggesting that CAD1 integrity is monitored by the plant immune system. We further demonstrate that CAD1 localizes to both the cytosol and plasma membrane using confocal microscopy and subcellular fractionation. Our results offer new insights into immune homeostasis and provide tools to further decipher the intriguing role of MACPF proteins in plants.

Item Type:	Article
Additional Information:	© The Author(s) 2021. Published by Oxford University Press on behalf of the Genetics Society of America. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Uncontrolled Keywords:	arabidopsis thaliana,cad1,mamp,immunity,membrane-attack complex,perforin,genetics ,/dk/atira/pure/subjectarea/asjc/1300/1311
Faculty \ School:	Faculty of Science > The Sainsbury Laboratory
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	19 Apr 2021 23:50
Last Modified:	23 Oct 2022 02:23
URI:	https://ueaeprints.uea.ac.uk/id/eprint/79823
DOI:	10.1093/genetics/iyab022

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