Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber

McGrath, Conor J. and Schuller, Stephanie (2021) Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber. In: Shiga Toxin-Producing E. coli: Methods and Protocols. Springer, pp. 273-283. ISBN 978-1-0716-1338-2

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Abstract

The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.

Item Type: Book Section
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
Depositing User: LivePure Connector
Date Deposited: 24 Mar 2021 00:45
Last Modified: 12 Jun 2021 00:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/79552
DOI: 10.1007/978-1-0716-1339-9_12

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