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McGrath, Conor J. and Schuller, Stephanie 
ORCID: https://orcid.org/0000-0003-3260-9112
(2021)
Determining Shiga toxin-producing Escherichia coli interactions with human intestinal epithelium in a microaerobic vertical diffusion chamber.
In:
Shiga Toxin-Producing E. coli: Methods and Protocols.
Springer, pp. 273-283.
ISBN 978-1-0716-1338-2
Abstract
The environment in the human intestine is low in oxygen. This affects virulence gene expression of enteropathogens including Shiga toxin-producing E. coli (STEC) and enables mucosal colonization by oxygen-sensitive commensal bacteria. To simulate the oxygen-restricted milieu at the intestinal epithelium, we have developed a vertical diffusion chamber model (VDC) which allows infection of polarized human intestinal epithelia under microaerobic conditions. In this chapter, we present a detailed protocol for performing STEC infections in the VDC system and subsequent analysis of STEC pathogenesis.
| Item Type: | Book Section |
|---|---|
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Mar 2021 00:45 |
| Last Modified: | 25 Sep 2024 10:43 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/79552 |
| DOI: | 10.1007/978-1-0716-1339-9_12 |
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