A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector

Witek, Kamil, Lin, Xiao, Karki, Hari S., Jupe, Florian, Witek, Agnieszka I., Steuernagel, Burkhard, Stam, Remco, Van Oosterhout, Cock, Fairhead, Sebastian, Heal, Robert, Cocker, Jonathan M., Bhanvadia, Shivani, Barrett, William, Wu, Chih-Hang, Adachi, Hiroaki, Song, Tianqiao, Kamoun, Sophien, Vleeshouwers, Vivianne G. A. A., Tomlinson, Laurence, Wulff, Brande B. H. and Jones, Jonathan D. G. (2021) A complex resistance locus in Solanum americanum recognizes a conserved Phytophthora effector. Nature Plants, 7 (2). pp. 198-208. ISSN 2055-026X

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Abstract

Late blight caused by Phytophthora infestans greatly constrains potato production. Many Resistance (R) genes were cloned from wild Solanum species and/or introduced into potato cultivars by breeding. However, individual R genes have been overcome by P. infestans evolution; durable resistance remains elusive. We positionally cloned a new R gene, Rpi-amr1, from Solanum americanum, that encodes an NRC helper-dependent CC-NLR protein. Rpi-amr1 confers resistance in potato to all 19 P. infestans isolates tested. Using association genomics and long-read RenSeq, we defined eight additional Rpi-amr1 alleles from different S. americanum and related species. Despite only ~90% identity between Rpi-amr1 proteins, all confer late blight resistance but differentially recognize Avramr1 orthologues and paralogues. We propose that Rpi-amr1 gene family diversity assists detection of diverse paralogues and alleles of the recognized effector, facilitating durable resistance against P. infestans.

Item Type: Article
Faculty \ School:
Faculty of Science > School of Environmental Sciences
Faculty of Science > School of Biological Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 19 Mar 2021 01:02
Last Modified: 27 Oct 2021 02:19
URI: https://ueaeprints.uea.ac.uk/id/eprint/79507
DOI: 10.1038/s41477-021-00854-9

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