Tools
Tools
Foster-Nyarko, Ebenezer, Alikhan, Nabil-Fareed, Ravi, Anuradha, Thomson, Nicholas M., Jarju, Sheikh, Kwambana-Adams, Brenda A., Secka, Arss, O'Grady, Justin, Antonio, Martin and Pallen, Mark John 
ORCID: https://orcid.org/0000-0003-1807-3657
(2021)
Genomic diversity of Escherichia coli isolates from backyard chickens and guinea fowl in the Gambia.
Microbial Genomics, 7 (1).
ISSN 2057-5858
Preview |
PDF (Published_Version)
- Published Version
Available under License Creative Commons Attribution. Download (2MB) | Preview |
Abstract
Chickens and guinea fowl are commonly reared in Gambian homes as affordable sources of protein. Using standard microbiological techniques, we obtained 68 caecal isolates of Escherichia coli from 10 chickens and 9 guinea fowl in rural Gambia. After Illumina whole-genome sequencing, 28 sequence types were detected in the isolates (4 of them novel), of which ST155 was the most common (22/68, 32 %). These strains span four of the eight main phylogroups of E. coli, with phylogroups B1 and A being most prevalent. Nearly a third of the isolates harboured at least one antimicrobial resistance gene, while most of the ST155 isolates (14/22, 64 %) encoded resistance to ≥3 classes of clinically relevant antibiotics, as well as putative virulence factors, suggesting pathogenic potential in humans. Furthermore, hierarchical clustering revealed that several Gambian poultry strains were closely related to isolates from humans. Although the ST155 lineage is common in poultry from Africa and South America, the Gambian ST155 isolates belong to a unique cgMLST cluster comprising closely related (38-39 alleles differences) isolates from poultry and livestock from sub-Saharan Africa - suggesting that strains can be exchanged between poultry and livestock in this setting. Continued surveillance of E. coli and other potential pathogens in rural backyard poultry from sub-Saharan Africa is warranted.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | backyard poultry,chickens,escherichia coli,genomic diversity,guinea fowl,st155,epidemiology,microbiology,molecular biology,genetics ,/dk/atira/pure/subjectarea/asjc/2700/2713 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 09 Feb 2021 01:03 |
| Last Modified: | 22 Oct 2022 08:34 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/79191 |
| DOI: | 10.1099/mgen.0.000484 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |