Evolution of regulatory networks associated with traits under selection in cichlids

Mehta, Tarang K., Koch, Christopher, Nash, Will, Knaack, Sara A., Sudhakar, Padhmanand, Olbei, Marton, Bastkowski, Sarah, Penso-Dolfin, Luca, Korcsmaros, Tamas, Haerty, Wilfried ORCID: https://orcid.org/0000-0003-0111-191X, Roy, Sushmita and Di-Palma, Federica (2021) Evolution of regulatory networks associated with traits under selection in cichlids. Genome Biology, 22 (1). ISSN 1465-6906

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Background Seminal studies of vertebrate protein evolution speculated that gene regulatory changes can drive anatomical innovations. However, very little is known about gene regulatory network (GRN) evolution associated with phenotypic effect across ecologically diverse species. Here we use a novel approach for comparative GRN analysis in vertebrate species to study GRN evolution in representative species of the most striking examples of adaptive radiations, the East African cichlids. We previously demonstrated how the explosive phenotypic diversification of East African cichlids can be attributed to diverse molecular mechanisms, including accelerated regulatory sequence evolution and gene expression divergence. Results To investigate these mechanisms across species at a genome-wide scale, we develop a novel computational pipeline that predicts regulators for co-extant and ancestral co-expression modules along a phylogeny, and candidate regulatory regions associated with traits under selection in cichlids. As a case study, we apply our approach to a well-studied adaptive trait—the visual system—for which we report striking cases of network rewiring for visual opsin genes, identify discrete regulatory variants, and investigate their association with cichlid visual system evolution. In regulatory regions of visual opsin genes, in vitro assays confirm that transcription factor binding site mutations disrupt regulatory edges across species and segregate according to lake species phylogeny and ecology, suggesting GRN rewiring in radiating cichlids. Conclusions Our approach reveals numerous novel potential candidate regulators and regulatory regions across cichlid genomes, including some novel and some previously reported associations to known adaptive evolutionary traits.

Item Type: Article
Uncontrolled Keywords: cichlid,co-expression,gene regulatory network,molecular evolution,opsin,ecology, evolution, behavior and systematics,genetics,cell biology ,/dk/atira/pure/subjectarea/asjc/1100/1105
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Medicine and Health Sciences > Norwich Medical School
Faculty of Science
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Depositing User: LivePure Connector
Date Deposited: 16 Jan 2021 00:54
Last Modified: 22 Oct 2022 07:39
URI: https://ueaeprints.uea.ac.uk/id/eprint/78202
DOI: 10.1186/s13059-020-02208-8

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