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Hernández, Marcela 
ORCID: https://orcid.org/0000-0002-1041-785X, Vera-Gargallo, Blanca, Calabi-Floody, Marcela, King, Gary M., Conrad, Ralf and Tebbe, Christoph C.
(2020)
Reconstructing genomes of carbon monoxide oxidisers in volcanic deposits including members of the class Ktedonobacteria.
Microorganisms, 8 (12).
ISSN 2076-2607
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Abstract
Microorganisms can potentially colonise volcanic rocks using the chemical energy in reduced gases such as methane, hydrogen (H2) and carbon monoxide (CO). In this study, we analysed soil metagenomes from Chilean volcanic soils, representing three different successional stages with ages of 380, 269 and 63 years, respectively. A total of 19 metagenome-assembled genomes (MAGs) were retrieved from all stages with a higher number observed in the youngest soil (1640: 2 MAGs, 1751: 1 MAG, 1957: 16 MAGs). Genomic similarity indices showed that several MAGs had amino-acid identity (AAI) values >50% to the phyla Actinobacteria, Acidobacteria, Gemmatimonadetes, Proteobacteria and Chloroflexi. Three MAGs from the youngest site (1957) belonged to the class Ktedonobacteria (Chloroflexi). Complete cellular functions of all the MAGs were characterised, including carbon fixation, terpenoid backbone biosynthesis, formate oxidation and CO oxidation. All 19 environmental genomes contained at least one gene encoding a putative carbon monoxide dehydrogenase (CODH). Three MAGs had form I coxL operon (encoding the large subunit CO-dehydrogenase). One of these MAGs (MAG-1957-2.1, Ktedonobacterales) was highly abundant in the youngest soil. MAG-1957-2.1 also contained genes encoding a [NiFe]-hydrogenase and hyp genes encoding accessory enzymes and proteins. Little is known about the Ktedonobacterales through cultivated isolates, but some species can utilise H2 and CO for growth. Our results strongly suggest that the remote volcanic sites in Chile represent a natural habitat for Ktedonobacteria and they may use reduced gases for growth.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | carbon monoxide dehydrogenase,ktedonobacteria,metagenome-assembled-genome,shotgun sequencing,volcanic soils,microbiology,virology,microbiology (medical) ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Environmental Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Molecular Microbiology |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 07 Jan 2021 01:01 |
| Last Modified: | 25 Sep 2024 15:14 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/78081 |
| DOI: | 10.3390/microorganisms8121880 |
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