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ToolsKato, Hiroaki, Onai, Kiyoshi, Abe, Akira, Shimizu, Motoki, Takagi, Hiroki, Tateda, Chika, Utsushi, Hiroe, Singkarabanit-Ogawa, Suthitar, Kitakura, Saeko, Ono, Erika, Zipfel, Cyril, Takano, Yoshitaka, Ishiura, Masahiro and Terauchi, Ryoehei (2020) Lumi-Map, a real-time luciferase bioluminescence screen of mutants combined with MutMap, reveals Arabidopsis genes involved in PAMP-triggered immunity. Molecular Plant-Microbe Interactions, 33 (12). 1366–1380. ISSN 0894-0282
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Abstract
Plants recognize pathogen-associated molecular patterns (PAMPs) to activate PAMP-triggered immunity (PTI). However, our knowledge of PTI signaling remains limited. In this report, we introduce Lumi-Map, a high-throughput platform for identifying causative single-nucleotide polymorphisms (SNPs) for studying PTI signaling components. In Lumi-Map, a transgenic reporter plant line is produced that contains a firefly luciferase (LUC) gene driven by a defense gene promoter, which generates luminescence upon PAMP treatment. The line is mutagenized and the mutants with altered luminescence patterns are screened by a high-throughput real-time bioluminescence monitoring system. Selected mutants are subjected to MutMap analysis, a whole-genome sequencingbased method of rapid mutation identification, to identify the causative SNP responsible for the luminescence pattern change. We generated nine transgenic Arabidopsis reporter lines expressing the LUC gene fused to multiple promoter sequences of defense-related genes. These lines generate luminescence upon activation of FLAGELLIN-SENSING 2 (FLS2) by flg22, a PAMP derived from bacterial flagellin. We selected the WRKY29-promoter reporter line to identify mutants in the signaling pathway downstream of FLS2. After screening 24,000 ethylmethanesulfonate-induced mutants of the reporter line, we isolated 22 mutants with altered WRKY29 expression upon flg22 treatment (abbreviated as awf mutants). Although five flg22-insensitive awf mutants harbored mutations in FLS2 itself, Lumi-Map revealed three genes not previously associated with PTI. Lumi-Map has the potential to identify novel PAMPs and their receptors as well as signaling components downstream of the receptors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | arabidopsis thaliana,elicitors,genetics and gene regulation,high-throughput phenotyping,luciferase reporter,mamps,mutmap,pamp-triggered immunity,plant defense mechanisms,receptor,physiology,agronomy and crop science ,/dk/atira/pure/subjectarea/asjc/1300/1314 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 14 Nov 2020 01:14 |
| Last Modified: | 22 Oct 2022 07:20 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/77687 |
| DOI: | 10.1094/MPMI-05-20-0118-TA |
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