A genome-wide survey of DNA methylation in hexaploid wheat

Gardiner, Laura-Jayne, Quinton-Tulloch, Mark, Olohan, Lisa, Price, Jonathan, Hall, Neil ORCID: https://orcid.org/0000-0003-2808-0009 and Hall, Anthony ORCID: https://orcid.org/0000-0002-1806-020X (2015) A genome-wide survey of DNA methylation in hexaploid wheat. Genome Biology, 16. ISSN 1474-760X

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Abstract

BACKGROUND: DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulfite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat. RESULTS: While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We correlate sub-genome-specific promoter methylation with decreased expression levels and show that altered growing temperature has a small effect on methylation state, identifying a small but functionally relevant set of methylated genes. Finally, we demonstrate long-term methylation maintenance using a comparison between the D sub-genome of hexaploid wheat and its progenitor Aegilops tauschii. CONCLUSIONS: We show that tri-genome methylation is highly conserved with the diploid wheat progenitor while sub-genome-specific methylation shows more variation.

Item Type:	Article
Uncontrolled Keywords:	dna methylation,dna transposable elements,diploidy,gene expression,genome, plant,polyploidy,promoter regions, genetic,genetics,temperature,genetics
Depositing User:	LivePure Connector
Date Deposited:	07 Nov 2020 01:10
Last Modified:	09 May 2024 10:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/77590
DOI:	10.1186/s13059-015-0838-3

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