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Grocott, Timothy 
ORCID: https://orcid.org/0000-0002-6321-401X, Lozano Velasco, Estefania, Mok, Gi Fay ORCID: https://orcid.org/0000-0002-5202-9062 and Münsterberg, Andrea E. ORCID: https://orcid.org/0000-0002-4577-4240
(2020)
The Pax6 master control gene initiates spontaneous retinal development via a self-organising Turing network.
Development, 147 (24).
ISSN 0950-1991
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Abstract
Understanding how complex organ systems are assembled from simple embryonic tissues is a major challenge. Across the animal kingdom a great diversity of visual organs are initiated by a 'master control gene' called Pax6, which is both necessary and sufficient for eye development. Yet precisely how Pax6 achieves this deeply homologous function is poorly understood. Using the chick as a model organism, we show that vertebrate Pax6 interacts with a pair of morphogen-coding genes, Tgfb2 and Fst, to form a putative Turing network, which we have computationally modelled. Computer simulations suggest that this gene network is sufficient to spontaneously polarise the developing retina, establishing the first organisational axis of the eye and prefiguring its further development. Our findings reveal how retinal self-organisation may be initiated independently of the highly ordered tissue interactions that help to assemble the eye in vivo These results help to explain how stem cell aggregates spontaneously self-organise into functional eye-cups in vitro We anticipate these findings will help to underpin retinal organoid technology, which holds much promise as a platform for disease modelling, drug development and regenerative therapies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | eye development,follistatin,pattern formation,pax6,self-organisation,tgfβ,molecular biology,developmental biology ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 07 Nov 2020 01:09 |
| Last Modified: | 21 Apr 2023 00:50 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/77573 |
| DOI: | 10.1242/dev.185827 |
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