Tools
Tools
Nolan, Laura M., Turnbull, Lynne, Katrib, Marilyn, Osvath, Sarah R., Losa, Davide, Lazenby, James J. and Whitchurch, Cynthia B. 
ORCID: https://orcid.org/0000-0003-2296-3791
(2020)
Pseudomonas aeruginosa is capable of natural transformation in biofilms.
Microbiology, 166 (10).
995–1003.
ISSN 1350-0872
Preview |
PDF (995_micro000956)
- Published Version
Available under License Creative Commons Attribution. Download (740kB) | Preview |
Abstract
Natural transformation is a mechanism that enables competent bacteria to acquire naked, exogenous DNA from the environment. It is a key process that facilitates the dissemination of antibiotic resistance and virulence determinants throughout bacterial populations. Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen that produces large quantities of extracellular DNA (eDNA) that is required for biofilm formation. P. aeruginosa has a remarkable level of genome plasticity and diversity that suggests a high degree of horizontal gene transfer and recombination but is thought to be incapable of natural transformation. Here we show that P. aeruginosa possesses homologues of all proteins known to be involved in natural transformation in other bacterial species. We found that P. aeruginosa in biofilms is competent for natural transformation of both genomic and plasmid DNA. Furthermore, we demonstrate that type-IV pili (T4P) facilitate but are not absolutely essential for natural transformation in P. aeruginosa .
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 30 Oct 2020 01:04 |
| Last Modified: | 27 Sep 2024 23:54 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/77467 |
| DOI: | 10.1099/mic.0.000956 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |