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ToolsLin, Xiao, Song, Tianqiao, Fairhead, Sebastian, Witek, Kamil, Jouet, Agathe, Jupe, Florian, Witek, Agnieszka I., Karki, Hari S., Vleeshouwers, Vivianne G. A. A., Hein, Ingo and Jones, Jonathan D. G. (2020) Identification of Avramr1 from Phytophthora infestans using long read and cDNA pathogen‐enrichment sequencing (PenSeq). Molecular Plant Pathology, 21 (11). pp. 1502-1512. ISSN 1464-6722
Full text not available from this repository. (Request a copy)Abstract
Potato late blight, caused by the oomycete pathogen Phytophthora infestans, significantly hampers potato production. Recently, a new Resistance to Phytophthora infestans (Rpi) gene, Rpi‐amr1, was cloned from a wild Solanum species, Solanum americanum. Identification of the corresponding recognized effector (Avirulence or Avr) genes from P. infestans is key to elucidating their naturally occurring sequence variation, which in turn informs the potential durability of the cognate late blight resistance. To identify the P. infestans effector recognized by Rpi‐amr1, we screened available RXLR effector libraries and used long read and cDNA pathogen‐enrichment sequencing (PenSeq) on four P. infestans isolates to explore the untested effectors. Using single‐molecule real‐time sequencing (SMRT) and cDNA PenSeq, we identified 47 highly expressed effectors from P. infestans, including PITG_07569, which triggers a highly specific cell death response when transiently coexpressed with Rpi‐amr1 in Nicotiana benthamiana, suggesting that PITG_07569 is Avramr1. Here we demonstrate that long read and cDNA PenSeq enables the identification of full‐length RXLR effector families and their expression profile. This study has revealed key insights into the evolution and polymorphism of a complex RXLR effector family that is associated with the recognition by Rpi‐amr1.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | avramr1,penseq,phytophthora infestans,rxlr effector,rpi-amr1,late blight,oomycete,molecular biology,agronomy and crop science,soil science,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1312 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Environmental Sciences Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 01 Oct 2020 23:59 |
| Last Modified: | 18 Aug 2023 02:31 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/77074 |
| DOI: | 10.1111/mpp.12987 |
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