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Lee, Matthew J., Mantell, Judith, Brown, Ian R., Fletcher, Jordan M., Verkade, Paul, Pickersgill, Richard W., Woolfson, Derek N., Frank, Stefanie and Warren, Martin J. 
ORCID: https://orcid.org/0000-0002-6028-6456
(2018)
De novo targeting to the cytoplasmic and luminal side of bacterial microcompartments.
Nature Communications, 9 (1).
ISSN 2041-1723
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Abstract
Bacterial microcompartments, BMCs, are proteinaceous organelles that encase a specific metabolic pathway within a semi-permeable protein shell. Short encapsulation peptides can direct cargo proteins to the lumen of the compartments. However, the fusion of such peptides to non-native proteins does not guarantee encapsulation and often causes aggregation. Here, we report an approach for targeting recombinant proteins to BMCs that utilizes specific de novo coiled-coil protein–protein interactions. Attachment of one coiled-coil module to PduA (a component of the BMC shell) allows targeting of a fluorescent protein fused to a cognate coiled-coil partner. This interaction takes place on the outer surface of the BMC. The redesign of PduA to generate an N-terminus on the luminal side of the BMC results in intact compartments to which proteins can still be targeted via the designed coiled-coil system. This study provides a strategy to display proteins on the surface or within the lumen of the BMCs.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Norwich Institute for Healthy Aging |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 28 Aug 2020 23:57 |
| Last Modified: | 21 Apr 2023 00:45 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/76708 |
| DOI: | 10.1038/s41467-018-05922-x |
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