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Suen, Kin Man, Braukmann, Fabian, Butler, Richard, Bensaddek, Dalila, Akay, Alper 
ORCID: https://orcid.org/0000-0001-6825-4443, Lin, Chi-Chuan, Milonaitytė, Dovilė, Doshi, Neel, Sapetschnig, Alexandra, Lamond, Angus, Ladbury, John Edward and Miska, Eric Alexander
(2020)
DEPS-1 is required for piRNA-dependent silencing and PIWI condensate organisation in Caenorhabditis elegans.
Nature Communications, 11.
ISSN 2041-1723
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Abstract
Membraneless organelles are sites for RNA biology including small non-coding RNA (ncRNA) mediated gene silencing. How small ncRNAs utilise phase separated environments for their function is unclear. We investigated how the PIWI-interacting RNA (piRNA) pathway engages with the membraneless organelle P granule in Caenorhabditis elegans. Proteomic analysis of the PIWI protein PRG-1 reveals an interaction with the constitutive P granule protein DEPS-1. DEPS-1 is not required for piRNA biogenesis but piRNA-dependent silencing: deps-1 mutants fail to produce the secondary endo-siRNAs required for the silencing of piRNA targets. We identify a motif on DEPS-1 which mediates a direct interaction with PRG-1. DEPS-1 and PRG-1 form intertwining clusters to build elongated condensates in vivo which are dependent on the Piwi-interacting motif of DEPS-1. Additionally, we identify EDG-1 as an interactor of DEPS-1 and PRG-1. Our study reveals how specific protein-protein interactions drive the spatial organisation and piRNA-dependent silencing within membraneless organelles.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Cells and Tissues |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 26 Aug 2020 00:03 |
| Last Modified: | 21 Apr 2023 00:45 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/76660 |
| DOI: | 10.1038/s41467-020-18089-1 |
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