Cloning of the rice Xo1 resistance gene and interaction of the Xo1 protein with the defense-suppressing Xanthomonas effector Tal2h

Read, Andrew, Hutin, Mathilde, Moscou, Matthew, Rinaldi, Fabio and Bogdanove, Adam (2020) Cloning of the rice Xo1 resistance gene and interaction of the Xo1 protein with the defense-suppressing Xanthomonas effector Tal2h. Molecular Plant-Microbe Interactions, 33 (10). pp. 1189-1195. ISSN 0894-0282

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Abstract

The Xo1 locus in the heirloom rice variety Carolina Gold Select confers resistance to bacterial leaf streak and bacterial blight, caused by Xanthomonas oryzae pv. oryzicola and X. oryzae pv. oryzae, respectively. Resistance is triggered by pathogen-delivered transcription activator-like effectors (TALEs) independent of their ability to activate transcription and is suppressed by truncated variants called truncTALEs, common among Asian strains. By transformation of the susceptible variety Nipponbare, we show that one of 14 nucleotide-binding, leucine-rich repeat (NLR) protein genes at the locus, with a zinc finger BED domain, is the Xo1 gene. Analyses of published transcriptomes revealed that the Xo1-mediated response is more similar to those mediated by two other NLR resistance genes than it is to the response associated with TALE-specific transcriptional activation of the executor resistance gene Xa23 and that a truncTALE dampens or abolishes activation of defense-associated genes by Xo1. In Nicotiana benthamiana leaves, fluorescently tagged Xo1 protein, like TALEs and truncTALEs, localized to the nucleus. And endogenous Xo1 specifically coimmunoprecipitated from rice leaves with a pathogen-delivered, epitope-tagged truncTALE. These observations suggest that suppression of Xo1-function by truncTALEs occurs through direct or indirect physical interaction. They further suggest that effector coimmunoprecipitation may be effective for identifying or characterizing other resistance genes.

Item Type: Article
Additional Information: Funding Information: Funding: This work was supported by the Plant Genome Research Program of the National Science Foundation (Division of Integrative Organismal Systems IOS-1444511 to A. J. Bogdanove), the National Institute of Food and Agriculture of the United States Department of Agriculture (2018-67011-28025 to A. C. Read), and the Gatsby Charitable Foundation (to M. J. Moscou). We also acknowledge support from the National Institutes of Health to the Proteomics Facility for the Orbitrap Fusion mass spectrometer (shared instrumentation grant 1S10 OD017992-01) and to the Imaging Facility for the Zeiss LSM 710 confocal microscope (shared instrumentation grant S10RR025502). Publisher Copyright: © 2020 The American Phytopathological Society
Uncontrolled Keywords: defense suppression,effectors,mass spectrometry,nucleotide binding leucine-rich repeat (nlr),protein-protein interaction,resistance genes,transcription activator-like effector (tale),trunctale,physiology,agronomy and crop science ,/dk/atira/pure/subjectarea/asjc/1300/1314
Faculty \ School:
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 25 Aug 2020 00:05
Last Modified: 05 Oct 2021 01:14
URI: https://ueaeprints.uea.ac.uk/id/eprint/76557
DOI: 10.1094/MPMI-05-20-0131-SC

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