Chessa, Daniela, Murgia, Manuela, Sias, Emanuela, Deligios, Massimo, Mazzarello, Vittorio, Fiamma, Maura, Rovina, Daniela, Carenti, Gabriele, Ganau, Giulia, Pintore, Elisabetta, Fiori, Mauro, Kay, Gemma L., Ponzeletti, Alessandro, Cappuccinelli, Piero, Kelvin, David J., Wain, John and Rubino, Salvatore (2020) Metagenomics and microscope revealed T. trichiura and other intestinal parasites in a cesspit of an Italian nineteenth century aristocratic palace. Scientific Reports, 10. ISSN 2045-2322
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Abstract
This study evidenced the presence of parasites in a cesspit of an aristocratic palace of nineteenth century in Sardinia (Italy) by the use of classical paleoparasitological techniques coupled with next-generation sequencing. Parasite eggs identified by microscopy included helminth genera pathogenic for humans and animals: the whipworm Trichuris sp., the roundworm Ascaris sp., the flatworm Dicrocoelium sp. and the fish tapeworm Diphyllobothrium sp. In addition, 18S rRNA metabarcoding and metagenomic sequencing analysis allowed the first description in Sardinia of aDNA of the human specific T. trichiura species and Ascaris genus. Their presence is important for understanding the health conditions, hygiene habits, agricultural practices and the diet of the local inhabitants in the period under study.
Item Type: | Article |
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Uncontrolled Keywords: | sdg 2 - zero hunger,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/zero_hunger |
Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School |
Related URLs: | |
Depositing User: | LivePure Connector |
Date Deposited: | 13 Aug 2020 23:58 |
Last Modified: | 31 Jan 2024 02:42 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/76431 |
DOI: | 10.1038/s41598-020-69497-8 |
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