Tools
ToolsTejera, Noemi, Crossman, Lisa, Pearson, Bruce, Stoakes, Emily, Nasher, Fauzy, Djeghout, Bilal, Poolman, Mark, Wain, John and Singh, Dipali (2020) Genome-scale metabolic model driven design of a defined medium for Campylobacter jejuni M1cam. Frontiers in Microbiology, 11. ISSN 1664-302X
Preview |
PDF (Published_Version)
- Published Version
Available under License Creative Commons Attribution. Download (659kB) | Preview |
Abstract
Campylobacter jejuni, the most frequent cause of food-borne bacterial gastroenteritis, is a fastidious organism when grown in the laboratory. Oxygen is required for growth, despite the presence of the metabolic mechanism for anaerobic respiration. Amino acid auxotrophies are variably reported and energy metabolism can occur through several electron donor/acceptor combinations. Overall, the picture is one of a flexible, but vulnerable metabolism. To understand Campylobacter metabolism, we have constructed a fully curated, metabolic model for the reference organism M1 (our variant is M1cam) and validated it through laboratory experiments. Our results show that M1cam is auxotrophic for methionine, niacinamide, and pantothenate. There are complete biosynthesis pathways for all amino acids except methionine and it can produce energy, but not biomass, in the absence of oxygen. M1cam will grow in DMEM/F-12 defined media but not in the previously published Campylobacter specific defined media tested. Using the model, we identified potential auxotrophies and substrates that may improve growth. With this information, we designed simple defined media containing inorganic salts, the auxotrophic substrates, L-methionine, niacinamide, and pantothenate, pyruvate and additional amino acids L-cysteine, L-serine, and L-glutamine for growth enhancement. Our defined media supports a 1.75-fold higher growth rate than Brucella broth after 48 h at 37°C and sustains the growth of other Campylobacter jejuni strains. This media can be used to design reproducible assays that can help in better understanding the adaptation, stress resistance, and the virulence mechanisms of this pathogen. We have shown that with a well-curated metabolic model it is possible to design a media to grow this fastidious organism. This has implications for the investigation of new Campylobacter species defined through metagenomics, such as C. infans.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | auxotrophy,campylobacter jejuni,defined growth media,genome-scale metabolic model,linear programming,metabolic network,metabolism,microbiology,microbiology (medical) ,/dk/atira/pure/subjectarea/asjc/2400/2404 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 17 Jul 2020 23:46 |
| Last Modified: | 22 Oct 2022 06:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/76167 |
| DOI: | 10.3389/fmicb.2020.01072 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |