Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine

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Mkrtchyan, Hermine, Gibbons, Simon, Heidelberger, Sibylle, Zloh, Mire and Limaki, Hamidreza Khalatbari (2010) Purification, characterisation and identification of acidocin LCHV, an antimicrobial peptide produced by Lactobacillus acidophilus n.v. Er 317/402 strain Narine. International Journal of Antimicrobial Agents, 35 (3). pp. 255-260. ISSN 0924-8579

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Abstract

In the last two decades, antimicrobial peptides (AMPs) have been gaining attention as antimicrobial alternatives to chemical food preservatives and commonly used antibiotics. Lactobacillus acidophilus n.v. Er 317/402 strain Narine produces a small AMP with a molecular weight of 1.1 kDa, designated acidocin LCHV. In this study, the AMP was extremely heat stable (90 min at 130 °C), was active over a wide pH range and was found to be sensitive to proteolytic enzymes (trypsin, pepsin and proteinase K). Acidocin LCHV has a broad spectrum of activity both against Gram-positive and Gram-negative pathogens, including several that are classified as Especially Dangerous Infections by the World Health Organization as well as meticillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile. Matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF/MS) was used to determine the molecular mass and sequence of the purified peptide. Complete killing with immediate impact on cells was observed within a very short period of time (10 min).

Item Type:	Article
Uncontrolled Keywords:	antimicrobial peptide,l. acidophilus,pathogenic microorganisms,probiotic,microbiology (medical),infectious diseases,pharmacology (medical),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2726
Faculty \ School:	Faculty of Science > School of Pharmacy
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	15 Jul 2020 23:39
Last Modified:	22 Oct 2022 06:29
URI:	https://ueaeprints.uea.ac.uk/id/eprint/76132
DOI:	10.1016/j.ijantimicag.2009.11.017

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