Tools
ToolsChoux, Cecile, Petazzi, Paolo, Sanchez-Delago, Marta, Hernandez Mora, Jose R., Monteagudo, Ana, Sagot, Paul, Monk, David and Fauque, Patricia (2020) The hypomethylation of imprinted genes in IVF/ICSI placenta samples is associated with concomitant changes in histone modifications. Epigenetics, 15 (12). pp. 1386-1395. ISSN 1559-2294
Preview |
PDF (Accepted_Manuscript)
- Accepted Version
Download (2MB) | Preview |
Abstract
Although more and more children are born by Assisted Reproductive Technologies (ART), ART safety has not fully been demonstrated. Notably, ART could disturb the delicate step of implantation, and trigger placenta-related adverse outcomes with potential long-term effects, through disrupted epigenetic regulation. We have previously demonstrated that placental DNA methylation was significantly lower after IVF/ICSI than following natural conception at two differentially methylated regions (DMRs) associated with imprinted genes (IGs): H19/IGF2 and KCNQ1OT1. As histone modifications are critical for placental physiology, the aim of this study was to profile permissive and repressive histone marks in placenta biopsies to reveal a better understanding of the epigenetic changes in the context of ART. Utilizing chromatin immunoprecipitation (ChIP) coupled with quantitative PCR, permissive (H3K4me3, H3K4me2, and H3K9ac) and repressive (H3K9me3 and H3K9me2) post-translational histone modifications were quantified. The analyses revealed a significantly higher quantity of H3K4me2 precipitation in the IVF/ICSI group than in the natural conception group for H19/IGF2 and KCNQ1OT1 DMRs (P = 0.016 and 0.003, respectively). Conversely, the quantity of both repressive marks at H19/IGF2 and SNURF DMRs was significantly lower in the IVF/ICSI group than in the natural conception group (P = 0.011 and 0.027 for H19/IGF2; and P = 0.010 and 0.035 for SNURF). These novel findings highlight that DNA hypomethylation at imprinted DMRs following ART is linked with increased permissive/decreased repressive histone marks, altogether promoting a more permissive chromatin conformation. This concomitant change in epigenetic state at IGs at birth might be an important developmental event because of ART manipulations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | assisted reproductive technologies,epigenetics,histone modifications,in vitro fertilization,dna methylation,placenta |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 09 Jul 2020 00:08 |
| Last Modified: | 22 Oct 2022 06:26 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/75979 |
| DOI: | 10.1080/15592294.2020.1783168 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |