Tools
Tools
Kiu, Raymond, Treveil, Agatha, Harnisch, Lukas C., Caim, Shabhonam, Leclaire, Charlotte, van Sinderen, Douwe, Korcsmaros, Tamas and Hall, Lindsay J. 
ORCID: https://orcid.org/0000-0001-8938-5709
(2020)
Bifidobacterium breve UCC2003 induces a distinct global transcriptomic programme in neonatal murine intestinal epithelial cells.
iScience, 23 (7).
ISSN 2589-0042
Preview |
PDF (Accepted_Manuscript)
- Accepted Version
Available under License Creative Commons Attribution. Download (4MB) | Preview |
Abstract
The underlying health-driving mechanisms of Bifidobacterium during early life are not well understood, particularly how this microbiota member may modulate the intestinal barrier via programming of intestinal epithelial cells (IECs). We investigated the impact of Bifidobacterium breve UCC2003 on the transcriptome of neonatal murine IECs. Small IECs from two-week-old neonatal mice administered B. breve UCC2003 or PBS (control) were subjected to global RNA-Seq, and differentially expressed genes, pathways and affected cell types determined. We observed extensive regulation of the IEC transcriptome with ∼4,000 genes significantly up-regulated, including key genes linked with epithelial barrier function. Enrichment of cell differentiation pathways were observed, along with an overrepresentation of stem cell marker genes, indicating an increase in the regenerative potential of the epithelial layer. In conclusion, B. breve UCC2003 plays a central role in driving intestinal epithelium homeostatic development during early life and suggests future avenues for next-stage clinical studies.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Science Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 07 Jul 2020 23:59 |
| Last Modified: | 22 Oct 2022 06:26 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/75954 |
| DOI: | 10.1016/j.isci.2020.101336 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |