Inhibiting plasmid mobility: The effect of isothiocyanates on bacterial conjugation

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Kwapong, Awo Afi, Stapleton, Paul and Gibbons, Simon (2019) Inhibiting plasmid mobility: The effect of isothiocyanates on bacterial conjugation. International Journal of Antimicrobial Agents, 53 (5). pp. 629-636. ISSN 0924-8579

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Abstract

Bacterial conjugation is the main mechanism for the transfer of multiple antimicrobial resistance genes among pathogenic micro-organisms. This process may be controlled by compounds that inhibit bacterial conjugation. In this study, the effects of allyl isothiocyanate, L-sulforaphane, benzyl isothiocyanate, phenylethyl isothiocyanate and 4-methoxyphenyl isothiocyanate on the conjugation of broad-host-range plasmids harbouring various antimicrobial resistance genes in Escherichia coli were investigated, namely plasmids pKM101 (IncN), TP114 (IncI 2 ), pUB307 (IncP) and the low-copy-number plasmid R7K (IncW). Benzyl isothiocyanate (32 mg/L) significantly reduced conjugal transfer of pKM101, TP114 and pUB307 to 0.3 ± 0.6%, 10.7 ± 3.3% and 6.5 ± 1.0%, respectively. L-sulforaphane (16 mg/L; transfer frequency 21.5 ± 5.1%) and 4-methoxyphenyl isothiocyanate (100 mg/L; transfer frequency 5.2 ± 2.8%) were the only compounds showing anti-conjugal specificity by actively reducing the transfer of R7K and pUB307, respectively.

Item Type: Article
Uncontrolled Keywords: bacterial conjugation,conjugative plasmid,effector protein,horizontal gene transfer,isothiocyanate,plasmid incompatibility group,microbiology (medical),infectious diseases,pharmacology (medical),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2700/2726
Faculty \ School: Faculty of Science > School of Pharmacy
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Depositing User: LivePure Connector
Date Deposited: 18 Jun 2020 00:02
Last Modified: 22 Oct 2022 06:19
URI: https://ueaeprints.uea.ac.uk/id/eprint/75641
DOI: 10.1016/j.ijantimicag.2019.01.011

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