An aphid RNA transcript migrates systemically within plants and is a virulence factor

Chen, Yazhou, Singh, Archana, Kaithakottil, Gemy G, Mathers, Thomas C, Gravino, Matteo, Mugford, Sam T, van Oosterhout, Cock, Swarbreck, David and Hogenhout, Saskia A (2020) An aphid RNA transcript migrates systemically within plants and is a virulence factor. Proceedings of the National Academy of Sciences of the United States of America, 117 (23). pp. 12763-12771. ISSN 1091-6490

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Abstract

Aphids are sap-feeding insects that colonize a broad range of plant species and often cause feeding damage and transmit plant pathogens, including bacteria, viruses, and viroids. These insects feed from the plant vascular tissue, predominantly the phloem. However, it remains largely unknown how aphids, and other sap-feeding insects, establish intimate long-term interactions with plants. To identify aphid virulence factors, we took advantage of the ability of the green peach aphid Myzus persicae to colonize divergent plant species. We found that a M. persicae clone of near-identical females established stable colonies on nine plant species of five representative plant eudicot and monocot families that span the angiosperm phylogeny. Members of the novel aphid gene family Ya are differentially expressed in aphids on the nine plant species and are coregulated and organized as tandem repeats in aphid genomes. Aphids translocate Ya transcripts into plants, and some transcripts migrate to distal leaves within several plant species. RNAi-mediated knockdown of Ya genes reduces M. persicae fecundity, and M. persicae produces more progeny on transgenic plants that heterologously produce one of the systemically migrating Ya transcripts as a long noncoding (lnc) RNA. Taken together, our findings show that beyond a range of pathogens, M. persicae aphids translocate their own transcripts into plants, including a Ya lncRNA that migrates to distal locations within plants, promotes aphid fecundity, and is a member of a previously undescribed host-responsive aphid gene family that operate as virulence factors.

Item Type: Article
Additional Information: The data reported in this paper have been deposited in the Gene Expression Omnibus (GEO) database, https://www.ncbi.nlm.nih.gov/geo under following accession GSE129669. This article contains supporting information online at https://www.pnas.org/lookup/suppl/doi:10.1073/pnas.1918410117/-/DCSupplemental.
Uncontrolled Keywords: aphids,lncrna,plant–insect interactions,trafficking,transkingdom rna,virulence,general ,/dk/atira/pure/subjectarea/asjc/1000
Faculty \ School: Faculty of Science > School of Biological Sciences
Faculty of Science > School of Environmental Sciences
Related URLs:
Depositing User: LivePure Connector
Date Deposited: 03 Jun 2020 00:13
Last Modified: 07 Jul 2020 23:57
URI: https://ueaeprints.uea.ac.uk/id/eprint/75448
DOI: 10.1073/pnas.1918410117

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