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Ellis, Samuel J., Crossman, Lisa C., McGrath, Conor J., Chattaway, Marie A., Hölken, Johanna M., Brett, Bernard, Bundy, Leah, Kay, Gemma L., Wain, John and Schüller, Stephanie 
ORCID: https://orcid.org/0000-0003-3260-9112
(2020)
Identification and characterisation of enteroaggregative Escherichia coli subtypes associated with human disease.
Scientific Reports, 10.
ISSN 2045-2322
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Abstract
Enteroaggregative E. coli (EAEC) are a major cause of diarrhoea worldwide. Due to their heterogeneity and carriage in healthy individuals, identification of diagnostic virulence markers for pathogenic strains has been difficult. In this study, we have determined phenotypic and genotypic differences between EAEC strains of sequence types (STs) epidemiologically associated with asymptomatic carriage (ST31) and diarrhoeal disease (ST40). ST40 strains demonstrated significantly enhanced intestinal adherence, biofilm formation, and pro-inflammatory interleukin-8 secretion compared with ST31 isolates. This was independent of whether strains were derived from diarrhoea patients or healthy controls. Whole genome sequencing revealed differences in putative virulence genes encoding aggregative adherence fimbriae, E. coli common pilus, flagellin and EAEC heat-stable enterotoxin 1. Our results indicate that ST40 strains have a higher intrinsic potential of human pathogenesis due to a specific combination of virulence-related factors which promote host cell colonization and inflammation. These findings may contribute to the development of genotypic and/or phenotypic markers for EAEC strains of high virulence.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | general,sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/1000 |
| Faculty \ School: | Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 16 May 2020 00:23 |
| Last Modified: | 11 Dec 2024 01:32 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/75176 |
| DOI: | 10.1038/s41598-020-64424-3 |
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