Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants

Cheval, Cécilia, Samwald, Sebastian, Johnston, Matthew G., de Keijzer, Jeroen, Breakspear, Andrew, Liu, Xiaokun, Bellandi, Annalisa, Kadota, Yasuhiro, Zipfel, Cyril and Faulkner, Christine (2020) Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants. Proceedings of the National Academy of Sciences of the United States of America, 117 (17). pp. 9621-9629. ISSN 1091-6490

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Abstract

The plasma membrane (PM) is composed of heterogeneous subdomains, characterized by differences in protein and lipid composition. PM receptors can be dynamically sorted into membrane domains to underpin signaling in response to extracellular stimuli. In plants, the plasmodesmal PM is a discrete microdomain that hosts specific receptors and responses. We exploited the independence of this PM domain to investigate how membrane domains can independently integrate a signal that triggers responses across the cell. Focusing on chitin signaling, we found that responses in the plasmodesmal PM require the LysM receptor kinases LYK4 and LYK5 in addition to LYM2. Chitin induces dynamic changes in the localization, association, or mobility of these receptors, but only LYM2 and LYK4 are detected in the plasmodesmal PM. We further uncovered that chitin-induced production of reactive oxygen species and callose depends on specific signaling events that lead to plasmodesmata closure. Our results demonstrate that distinct membrane domains can integrate a common signal with specific machinery that initiates discrete signaling cascades to produce a localized response.

Item Type:	Article
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > The Sainsbury Laboratory
UEA Research Groups:	Faculty of Science > Research Groups > Plant Sciences
Depositing User:	LivePure Connector
Date Deposited:	01 May 2020 00:06
Last Modified:	17 Oct 2024 00:21
URI:	https://ueaeprints.uea.ac.uk/id/eprint/74926
DOI:	10.1073/pnas.1907799117

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