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Püngel, Deborah, Treveil, Agatha, Dalby, Matthew J., Caim, Shabhonam, Colquhoun, Ian J., Booth, Catherine, Ketskemety, Jennifer, Korcsmaros, Tamas, van Sinderen, Douwe, Lawson, Melissa A. E. and Hall, Lindsay J. 
ORCID: https://orcid.org/0000-0001-8938-5709
(2020)
Bifidobacterium breve UCC2003 exopolysaccharide modulates the early life microbiota by acting as a potential dietary substrate.
Nutrients, 12 (4).
ISSN 2072-6643
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Abstract
Background: Bifidobacterium represents an important early life microbiota member. Specific bifidobacterial components, exopolysaccharides (EPS), positively modulate host responses, with purified EPS also suggested to impact microbe–microbe interactions by acting as a nutrient substrate. Thus, we determined the longitudinal effects of bifidobacterial EPS on microbial communities and metabolite profiles using an infant model colon system. Methods: Differential gene expression and growth characteristics were determined for each strain; Bifidobacterium breve UCC2003 and corresponding isogenic EPS-deletion mutant (B. breve UCC2003del). Model colon vessels were inoculated with B. breve and microbiome dynamics monitored using 16S rRNA sequencing and metabolomics (NMR). Results: Transcriptomics of EPS mutant vs. B. breve UCC2003 highlighted discrete differential gene expression (e.g., eps biosynthetic cluster), though overall growth dynamics between strains were unaffected. The EPS-positive vessel had significant shifts in microbiome and metabolite profiles until study end (405 h); with increases of Tyzzerella and Faecalibacterium, and short-chain fatty acids, with further correlations between taxa and metabolites which were not observed within the EPS-negative vessel. Conclusions: These data indicate that B. breve UCC2003 EPS is potentially metabolized by infant microbiota members, leading to differential microbial metabolism and altered metabolite by-products. Overall, these findings may allow development of EPS-specific strategies to promote infant health.
| Item Type: | Article |
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| Additional Information: | Funding: This work was part funded by an Erasmus studentship to D.P. M.A.E.L. was funded by the Marie Skłodowska-Curie Individual Fellowship (Project 661594). L.J.H. is funded by a Wellcome Trust Investigator award (100974/C/13/Z) and together with T.K. by a BBSRC ISP grant for Gut Microbes and Health BB/R012490/1 and its constituent project(s), BBS/E/F/000PR10353 and BBS/E/F/000PR10355. T.K. is also funded by the Genomics for Food security CSP grant from the BBSRC (BB/CSP17270/1). A.T. is supported by the BBSRC Norwich Research Park Biosciences Doctoral Training Partnership (grant BB/M011216/1). D.v.S. is supported by Science Foundation Ireland (SFI/12/RC/2273-P1 and SFI/12/RC/2273-P2). The funding bodies did not contribute to the design of the study, collection, analysis, and interpretation of data or in writing the manuscript. |
| Uncontrolled Keywords: | 16s rrna profiling,bifidobacterium,cross-feeding,diet,early life,exopolysaccharides,metabolomics,model colon,food science,nutrition and dietetics ,/dk/atira/pure/subjectarea/asjc/1100/1106 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences Faculty of Medicine and Health Sciences > Norwich Medical School |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 07 Apr 2020 00:44 |
| Last Modified: | 22 Oct 2022 06:00 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/74717 |
| DOI: | 10.3390/nu12040948 |
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