Tools
Tools
Anderson, Michelle, Purcell, Jessica, Verkuijl, Sebald, Norman, Victoria, Leftwich, Philip 
ORCID: https://orcid.org/0000-0001-9500-6592, Harvey-Samuel, Tim and Alphey, Luke
(2020)
Expanding the CRISPR toolbox in Culicine mosquitoes: in vitro validation of Pol III promoters.
ACS Synthetic Biology, 9 (3).
pp. 678-681.
Preview |
PDF (Published_Version)
- Published Version
Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
CRISPR–Cas9-based “gene drive” technologies have been proposed as a novel and effective means of controlling human diseases vectored by mosquitoes. However, more complex designs than those demonstrated to date—and an expanded molecular toolbox with which to build them—will be required to overcome the issues of resistance formation/evolution and drive spatial/temporal limitation. Foreseeing this need, we assessed the sgRNA transcriptional activities of 33 phylogenetically diverse insect Polymerase III promoters using three disease-relevant Culicine mosquito cell lines (Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus). We show that U6 promoters work across species with a range of transcriptional activity levels and find 7SK promoters to be especially promising because of their broad phylogenetic activity. We further show that U6 promoters can be substantially truncated without affecting transcriptional levels. These results will be of great utility to researchers involved in developing the next generation of gene drives.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 7sk promoter,cas9,polymerase iii,u6 promoter,gene drive,mosquito,biomedical engineering,biochemistry, genetics and molecular biology (miscellaneous),sdg 3 - good health and well-being ,/dk/atira/pure/subjectarea/asjc/2200/2204 |
| Faculty \ School: | Faculty of Science > School of Biological Sciences |
| UEA Research Groups: | Faculty of Science > Research Groups > Biosciences Teaching and Education Research |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 13 Mar 2020 10:51 |
| Last Modified: | 21 Apr 2023 00:26 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/74489 |
| DOI: | 10.1021/acssynbio.9b00436 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |