Stem rust resistance in wheat is suppressed by a subunit of the mediator complex

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Hiebert, Colin W., Moscou, Matthew J. ORCID: https://orcid.org/0000-0003-2098-6818, Hewitt, Tim, Steuernagel, Burkhard, Hernandez-Pinzon Toscano, Inmaculada, Green, Phon, Pujol, Vincent, Zhang, Peng, Rouse, Matthew N., Jin, Yue, McIntosh, Robert A., Upadhyaya, Narayana, Zhang, Jianping, Bhavani, Sridhar, Vrána, Jan, Karafiátová, Miroslava, Huang, Li, Fetch, Tom, Doležel, Jaroslav, Wulff, Brande B. H., Lagudah, Evans and Spielmeyer, Wolfgang (2020) Stem rust resistance in wheat is suppressed by a subunit of the mediator complex. Nature Communications, 11 (1). ISSN 2041-1723

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Abstract

Stem rust is an important disease of wheat that can be controlled using resistance genes. The gene SuSr-D1 identified in cultivar 'Canthatch' suppresses stem rust resistance. SuSr-D1 mutants are resistant to several races of stem rust that are virulent on wild-type plants. Here we identify SuSr-D1 by sequencing flow-sorted chromosomes, mutagenesis, and map-based cloning. The gene encodes Med15, a subunit of the Mediator Complex, a conserved protein complex in eukaryotes that regulates expression of protein-coding genes. Nonsense mutations in Med15b.D result in expression of stem rust resistance. Time-course RNAseq analysis show a significant reduction or complete loss of differential gene expression at 24h post inoculation in med15b.D mutants, suggesting that transcriptional reprogramming at this time point is not required for immunity to stem rust. Suppression is a common phenomenon and this study provides novel insight into suppression of rust resistance in wheat. Stem rust is an important disease of wheat and resistance present in some cultivars can be suppressed by the SuSr-D1 locus. Here the authors show that SuSr-D1 encodes a subunit of the Mediator Complex and that nonsense mutations are sufficient to abolish suppression and confer stem rust resistance.

Item Type: Article
Uncontrolled Keywords: f-sp tritici,aegilops-tauschii coss.,defense gene-expression,leaf rust,hexaploid wheat,common wheat,phylogenetic analysis,stripe rust,aestivum l,race ttks,physics and astronomy(all),chemistry(all),biochemistry, genetics and molecular biology(all) ,/dk/atira/pure/subjectarea/asjc/3100
Faculty \ School: Faculty of Science > The Sainsbury Laboratory
Faculty of Science > School of Biological Sciences
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Depositing User: LivePure Connector
Date Deposited: 03 Mar 2020 09:10
Last Modified: 16 Dec 2024 01:31
URI: https://ueaeprints.uea.ac.uk/id/eprint/74401
DOI: 10.1038/s41467-020-14937-2

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