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ToolsThomas, Paul, Wong, J. G., Lee, A. K. and Almers, W. (1993) A low affinity Ca2+ receptor controls the final steps in peptide secretion from pituitary melanotrophs. Neuron, 11 (1). pp. 93-104. ISSN 0896-6273
Full text not available from this repository.Abstract
Using flash photolysis of caged Ca2+ and the membrane capacitance to monitor exocytosis, we have studied the response of single melanotrophs to a step rise in cytosolic Ca2+ concentration ([Ca2+]i). Exocytosis begins with a rapid burst. This burst is followed by a slower phase, which is inhibited at cytosolic pH 6.2, and an ultraslow phase, which is strongly temperature sensitive. The exocytic burst starts with a delay of 6–11 ms and continues at a rate that grows steeply with [Ca2+]i and is half-maximal at [Ca2+]i = 27 μM. At least 3 Ca2+ ions are required to trigger exocytosis. The rate constant at saturating [Ca2+]i suggests that exocytosis of a dense core vesicle takes 40 ms after all Ca2+ ions have bound to their regulatory sites. If docked dense core vesicles cause the exocytic burst, they must decorate the plasma membrane at a mean density of 0.5/μm2.
| Item Type: | Article |
|---|---|
| Faculty \ School: | Faculty of Science |
| Depositing User: | LivePure Connector |
| Date Deposited: | 03 Mar 2020 09:09 |
| Last Modified: | 22 Oct 2022 05:54 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/74381 |
| DOI: | 10.1016/0896-6273(93)90274-U |
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