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ToolsAbdelhamid, Mahmoud and Waller, Zoe (2020) Tricky topology: Persistence of folded human telomeric i-motif DNA at ambient temperature and neutral pH. Frontiers in Chemistry, 8. ISSN 2296-2646
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Abstract
i-Motifs are four-stranded DNA structures formed from sequences rich in cytosine, held together by hemi-protonated cytosine-cytosine base pairs. These structures have been utilized extensively as pH-switches in DNA-based nanotechnology. Recently there has been an increasing interest in i-motif structures in biology, fuelled by examples of when these can form under neutral conditions. Herein we describe a cautionary tale regarding handling of i-motif samples. Using CD and UV spectroscopy we show that it is important to be consistent in annealing i-motif DNA samples as at neutral pH, i-motif unfolding kinetics is dependent on the time allowed for annealing and equilibration. We describe how the quadruplex structure formed by the human telomeric i-motif sequence can be shown to form and persist in the same conditions of neutral pH and ambient temperature in which, once at thermodynamic equilibrium, it exists predominantly as a random coil. This study has implications not only for work with i-motif DNA structures, but also in the uses and applications of these in nanotechnological devices.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | i-motif,cytosine,quadruplex,dna,topology,kinetics |
| Faculty \ School: | Faculty of Science > School of Pharmacy (former - to 2024) |
| UEA Research Groups: | Faculty of Science > Research Groups > Chemical Biology and Medicinal Chemistry (former - to 2021) |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 12 Feb 2020 05:38 |
| Last Modified: | 25 Sep 2024 14:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/74158 |
| DOI: | 10.3389/fchem.2020.00040 |
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