Quantum dot interactions with and toxicity to Shewanella oneidensis MR-1

Wroblewska-Wolna, Anna, Harvie, Andrew, Rowe, Sam, Critchley, Kevin, Butt, Julea ORCID: https://orcid.org/0000-0002-9624-5226 and Jeuken, Lars J. C. (2020) Quantum dot interactions with and toxicity to Shewanella oneidensis MR-1. Nanotechnology, 31 (13). ISSN 0957-4484

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Abstract

Combining abiotic photosensitisers such as quantum dots (QDs) with non-photosynthetic bacteria presents an intriguing concept into the design of artificial photosynthetic organisms and solar-driven fuel production. Shewanella oneidensis MR-1 (MR-1) is a versatile bacterium concerning respiration, metabolism and biocatalysis, and is a promising organism for artificial photosynthesis as the bacterium’s synthetic and catalytic ability provides a potential system for bacterial biohydrogen production. MR-1’s hydrogenases are present in the periplasmatic space. It follows that for photoenergised electrons to reach these enzymes, QDs will need to be able to enter the periplasm, or electrons need to enter the periplasm via the Mtr pathway that is responsible for MR-1’s extracellular electron transfer ability. As a step towards this goal, various QDs were tested for their photo-reducing potential, nanotoxicology and further for their interaction with MR-1. CdTe/CdS/TGA, CdTe/CdS/Cysteamine, a commercial, negatively charged CdTe and CuInS2/ZnS/PMAL QDs were examined. The photoreduction potential of the QDs was confirmed by measuring their ability to photoreduce methyl viologen with different sacrificial electron donors. The commercial CdTe and CuInS2/ZnS/PMAL QDs showed no toxicity towards MR-1 as evaluated by a colony-forming units method and a fluorescence viability assay. Only the commercial negatively charged CdTe QDs showed good interaction with MR-1. With transmission electron microscopy, QDs were observed both in the cytoplasm and periplasm. These results inform on the possibilities and bottlenecks when developing bionanotechnological systems for the photosynthetic production of biohydrogen by MR-1.

Item Type:	Article
Uncontrolled Keywords:	catalysis,cdte,electron-transfer,energy-transfer,extinction coefficient,gold nanoparticles,inorganic nanoparticles,nanocrystals,oxidative stress,oxide nanoparticles,artificial photosynthesis,bacteria,light harvesting,quantum dots,toxicity
Faculty \ School:	Faculty of Science > School of Biological Sciences Faculty of Science > School of Chemistry
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Energy Materials Laboratory
Related URLs:	http://www.scopus.com/inward/record.url?...
Depositing User:	LivePure Connector
Date Deposited:	05 Dec 2019 02:13
Last Modified:	22 Oct 2022 05:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/73268
DOI:	10.1088/1361-6528/ab5f78

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