The Salmonella specific, σE-regulated, STM1250 and AgsA, function with the sHsps IbpA and IbpB, to counter oxidative stress and survive macrophage killing

Tools

Hews, Claire L., Pritchard, Emily J. and Rowley, Gary ORCID: https://orcid.org/0000-0002-5421-4333 (2019) The Salmonella specific, σE-regulated, STM1250 and AgsA, function with the sHsps IbpA and IbpB, to counter oxidative stress and survive macrophage killing. Frontiers in Cellular and Infection Microbiology, 9. ISSN 2235-2988

[thumbnail of Published_Version]
Preview
 PDF (Published_Version) - Published Version
Available under License Creative Commons Attribution.
Download (2MB) | Preview

Abstract

The host presents an array of environments which induce bacterial stress including changes in pH, antimicrobial compounds and reactive oxygen species. The bacterial envelope sits at the interface between the intracellular and extracellular environment and its maintenance is essential for Salmonella cell viability under a range of conditions, including during infection. In this study, we aimed to understand the contribution of the σH- and σE-regulated small heat shock proteins IbpA, IbpB, and AgsA and the putative σE-regulated stress response protein STM1250 to the Salmonella envelope stress response. Due to shared sequence identity, regulatory overlap, and the specificity of STM1250 and AgsA to Salmonella sp., we hypothesized that functional overlap exists between these four stress response proteins, which might afford a selective advantage during Salmonella exposure to stress. We present here new roles for three small heat shock proteins and a putative stress response protein in Salmonella that are not limited to heat shock. We have shown that, compared to WT, a quadruple mutant is significantly more sensitive to hydrogen peroxide, has a lower minimum bactericidal concentration to the cationic antimicrobial peptide polymyxin B, and is attenuated in macrophages.

Item Type: Article
Faculty \ School: Faculty of Science > School of Biological Sciences
UEA Research Groups: Faculty of Science > Research Groups > Molecular Microbiology
Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group
Depositing User: LivePure Connector
Date Deposited: 11 Jul 2019 14:30
Last Modified: 25 Sep 2024 14:12
URI: https://ueaeprints.uea.ac.uk/id/eprint/71723
DOI: 10.3389/fcimb.2019.00263

Downloads

Downloads per month over past year

Actions (login required)

View Item View Item