Stiffness post-total knee replacement: a proof of principle study investigating the effect of gene expression analysis of markers of fibrosis:Gene expression of stiffness post-TKR

Mann, Charles J., Bradley, Anne M., Jones, Eleanor R., McNamara, Iain R., Smith, Toby O. ORCID: https://orcid.org/0000-0003-1673-2954 and Riley, Graham P. ORCID: https://orcid.org/0000-0001-5528-5611 (2019) Stiffness post-total knee replacement: a proof of principle study investigating the effect of gene expression analysis of markers of fibrosis:Gene expression of stiffness post-TKR. The Knee, 26 (4). pp. 914-922. ISSN 0968-0160

Preview

PDF (Accepted_Manuscript) - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. Download (1MB) | Preview

Abstract

BACKGROUND: To establish proof of principle of a link between phenotypic expression and stiffness after TKR. METHODS: From 100 patients, genetic expression of markers of fibrosis were performed for 15 synovial samples from patients categorised as ‘best post-operative range of movement (ROM)’ and 15 samples from patients with ‘worst ROM’. These markers included Matrix Metalloproteinases (MMPs), A Disintegrin and Metalloproteinases with Thrombospondin (ADAMTs) and Tissue Inhibitors of Matrix Metalloproteinases (TIMPs). Genetic marker data were compared to Oxford Knee Scores (OKS) and Pain Catastrophizing Scores (PCS). RESULTS: Quantitative markers for gene expression demonstrated more outliers in stiff compared to non-stiff knees, suggesting a greater imbalance in pro- and anti-fibrotic markers in stiff knees. Whilst there was a significant difference in the range of post-operative knee flexion (p=0.001) and extension (p=0.001), there was no statistically significant difference between stiff and non-stiff knees in pre-operative or post-operative OKS (p≥0.06). There was no difference in the individual components of the individual PCS score items nor the PCS total scores when stiff and non-stiff knees were compared (p>0.05). CONCLUSION: Biological factors, namely gene expression of MMPs, TIMPs and ADAMTs, may contribute towards post-TKR stiffness. This now warrants further investigation to better understand this relationship based on larger, multi-centre, cohorts.

Item Type:	Article
Uncontrolled Keywords:	knee arthroplasty,limited range,arthrofibrosis,gene expression,case-control
Faculty \ School:	Faculty of Medicine and Health Sciences > School of Health Sciences Faculty of Science > School of Biological Sciences
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Health Promotion Faculty of Medicine and Health Sciences > Research Groups > Musculoskeletal Medicine Faculty of Science > Research Groups > Cells and Tissues Faculty of Science > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Groups > Norwich Epidemiology Centre Faculty of Medicine and Health Sciences > Research Centres > Population Health
Depositing User:	LivePure Connector
Date Deposited:	08 May 2019 09:30
Last Modified:	19 Oct 2023 02:26
URI:	https://ueaeprints.uea.ac.uk/id/eprint/70868
DOI:	10.1016/j.knee.2019.05.005

Downloads

Actions (login required)

View Item