Tools
ToolsHarkins, Catriona P., Pettigrew, Kerry A., Oravcová, Katarina, Gardner, June, Hearn, R. M. Ross, Rice, Debbie, Mather, Alison E., Parkhill, Julian, Brown, Sara J., Proby, Charlotte M. and Holden, Matthew T. G. (2018) The microevolution and epidemiology of Staphylococcus aureus colonization during atopic eczema disease flare. Journal of Investigative Dermatology, 138 (2). pp. 336-343. ISSN 0022-202X
Preview |
PDF (Published_Version)
- Published Version
Available under License Creative Commons Attribution. Download (415kB) | Preview |
Abstract
Staphylococcus aureus is an opportunistic pathogen and variable component of the human microbiota. A characteristic of atopic eczema (AE) is colonization by S. aureus, with exacerbations associated with an increased bacterial burden of the organism. Despite this, the origins and genetic diversity of S. aureus colonizing individual patients during AE disease flares is poorly understood. To examine the microevolution of S. aureus colonization, we deep sequenced S. aureus populations from nine children with moderate to severe AE and 18 non-atopic children asymptomatically carrying S. aureus nasally. Colonization by clonal S. aureus populations was observed in both AE patients and control participants, with all but one of the individuals carrying colonies belonging to a single sequence type. Phylogenetic analysis showed that disease flares were associated with the clonal expansion of the S. aureus population, occurring over a period of weeks to months. There was a significant difference in the genetic backgrounds of S. aureus colonizing AE cases versus controls (Fisher exact test, P = 0.03). Examination of intra-host genetic heterogeneity of the colonizing S. aureus populations identified evidence of within-host selection in the AE patients, with AE variants being potentially selectively advantageous for intracellular persistence and treatment resistance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being |
| Faculty \ School: | |
| UEA Research Groups: | Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 24 Apr 2019 09:30 |
| Last Modified: | 06 Jun 2024 15:05 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/70649 |
| DOI: | 10.1016/j.jid.2017.09.023 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |