Carbapenem resistance in gram-negative bacteria in south-western Nigeria:The role of extended-spectrum β-lactamase CTX-M-15

Ogbolu, D. O. and Webber, M. A. (2018) Carbapenem resistance in gram-negative bacteria in south-western Nigeria:The role of extended-spectrum β-lactamase CTX-M-15. West Indian Medical Journal, 67 (4). pp. 344-349. ISSN 0043-3144

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Abstract

Objective: To determine the role of extended-spectrum β-lactamases in carbapenem-resistant Gram-negative bacteria from south-western Nigeria. Methods: Twenty-seven carbapenem-resistant isolates that were found to be non-carbapenemase producers (15 Escherichia coli, 9 Klebsiella pneumoniae and 3 Pseudomonas aeruginosa) were further studied. These isolates were subjected to analysis including phenotypic and genotypic detection of various β-lactamases, efflux activity, outer membrane protein, plasmids replicon typing, detection of transferable genes and resistances and typing using random amplified polymorphic DNA tests. Results: No isolates demonstrated de-repression of efflux, but all showed either complete loss or reduced production of outer membrane proteins. Transconjugants from these strains contained various genes including plasmid-mediated quinolone resistance and extended-spectrum beta-lactamases. All the transconjugants carried the blaCTX-M-15 gene. The transconjugants had varying minimum inhibitory concentrations of carbapenems ranging from 0.03 µg/ml to 8 µg/ml. Varying resistances to other antimicrobial agents were also transferred with the plasmids. The donor isolates were not clonally related by molecular typing. Conclusion: Resistance to carbapenem antibiotics in this sample was not mediated only by carbapenemases but also by production of extended-spectrum β-lactamases (largely CTX-M-15), accompanied by protein loss. This was an important mechanism underpinning carbapenem resistance in these clinical isolates of various species.

Item Type: Article
Uncontrolled Keywords: carbapenem,extended-spectrum beta-lactamase,gram-negative bacteria,nigeria,resistance,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700
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Depositing User: LivePure Connector
Date Deposited: 15 Apr 2019 14:30
Last Modified: 20 May 2020 00:02
URI: https://ueaeprints.uea.ac.uk/id/eprint/70579
DOI: 10.7727/wimj.2017.122

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