Tools
ToolsOgbolu, D. O. and Webber, M. A. (2018) Carbapenem resistance in gram-negative bacteria in south-western Nigeria:The role of extended-spectrum β-lactamase CTX-M-15. West Indian Medical Journal, 67 (4). pp. 344-349. ISSN 0043-3144
Full text not available from this repository.Abstract
Objective: To determine the role of extended-spectrum β-lactamases in carbapenem-resistant Gram-negative bacteria from south-western Nigeria. Methods: Twenty-seven carbapenem-resistant isolates that were found to be non-carbapenemase producers (15 Escherichia coli, 9 Klebsiella pneumoniae and 3 Pseudomonas aeruginosa) were further studied. These isolates were subjected to analysis including phenotypic and genotypic detection of various β-lactamases, efflux activity, outer membrane protein, plasmids replicon typing, detection of transferable genes and resistances and typing using random amplified polymorphic DNA tests. Results: No isolates demonstrated de-repression of efflux, but all showed either complete loss or reduced production of outer membrane proteins. Transconjugants from these strains contained various genes including plasmid-mediated quinolone resistance and extended-spectrum beta-lactamases. All the transconjugants carried the blaCTX-M-15 gene. The transconjugants had varying minimum inhibitory concentrations of carbapenems ranging from 0.03 µg/ml to 8 µg/ml. Varying resistances to other antimicrobial agents were also transferred with the plasmids. The donor isolates were not clonally related by molecular typing. Conclusion: Resistance to carbapenem antibiotics in this sample was not mediated only by carbapenemases but also by production of extended-spectrum β-lactamases (largely CTX-M-15), accompanied by protein loss. This was an important mechanism underpinning carbapenem resistance in these clinical isolates of various species.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | carbapenem,extended-spectrum beta-lactamase,gram-negative bacteria,nigeria,resistance,medicine(all) ,/dk/atira/pure/subjectarea/asjc/2700 |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 15 Apr 2019 14:30 |
| Last Modified: | 22 Oct 2022 04:34 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/70579 |
| DOI: | 10.7727/wimj.2017.122 |
Actions (login required)
 |
View Item |