Mutiga, S. K., Rotich, F., Ganeshan, V. Devi, Mwongera, D. T., Mgonja, E. M., Were, V. M., Harvey, J. W., Zhou, B., Wasilwa, L., Feng, C., Ouedraogo, I., Wang, G. -L., Mitchell, T. K., Talbot, N. J. ORCID: https://orcid.org/0000-0001-6434-7757 and Correll, J. C.
(2017)
Assessment of the virulence spectrum and its association with genetic diversity in Magnaporthe oryzae populations from sub-Saharan Africa.
Phytopathology, 107 (7).
pp. 852-863.
ISSN 0031-949X
Abstract
A collection of 122 isolates of Magnaporthe oryzae, from nine sub-Saharan African countries, was assessed for virulence diversity and genetic relatedness. The virulence spectrum was assessed by pathotype analysis with a panel of 43 rice genotypes consisting of differential lines carrying 24 blast resistance genes (R-genes), contemporary African rice cultivars, and susceptible checks. The virulence spectrum among isolates ranged from 5 to 80%. Five isolates were avirulent to the entire rice panel, while two isolates were virulent to similar to 75% of the panel. Overall, cultivar 75-1-127, the Pi9 R-gene donor, was resistant to all isolates (100%), followed by four African rice cultivars (AR105, NERICA 15, 96%; NERICA 4, 91%; and F6-36, 90%). Genetic relatedness of isolates was assessed by single nucleotide polymorphisms derived from genotyping-by-sequencing and by vegetative compatibility tests. Phylogenetic analysis of SNPs of a subset of isolates (n = 78) revealed seven distinct clades that differed in virulence. Principal component analysis showed isolates from East Africa were genetically distinct from those from West Africa. Vegetative compatibility tests of a subset of isolates (n = 65) showed no common groups among countries. This study shows that blast disease could be controlled by pyramiding of Pi9 together with other promising R-genes into rice cultivars that are adapted to East and West African regions.
Item Type: | Article |
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Uncontrolled Keywords: | resistance breeding,rice blast,virulence spectrum,rice blast fungus,vegetative compatibility,pyricularia-grisea,fusarium-oxysporum,resistance,pathogenicity,variability,site |
Faculty \ School: | Faculty of Science > The Sainsbury Laboratory Faculty of Science > School of Pharmacy |
Depositing User: | LivePure Connector |
Date Deposited: | 15 Feb 2019 10:30 |
Last Modified: | 21 Oct 2022 21:38 |
URI: | https://ueaeprints.uea.ac.uk/id/eprint/69939 |
DOI: | 10.1094/PHYTO-08-16-0319-R |
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