Characterization of evolutionarily conserved Trypanosoma cruzi NatC and NatA- N-terminal acetyltransferase complexes

Ochaya, Stephen, Franzen, Oscar, Buhwa, Doreen, Foyn, Havard, Butler, Claire, Stove, Svein, Tyler, Kevin ORCID: https://orcid.org/0000-0002-0647-8158, Arnesen, Thomas, Matovu, Enock, Aslund, Lena and Andersson, Björn (2019) Characterization of evolutionarily conserved Trypanosoma cruzi NatC and NatA- N-terminal acetyltransferase complexes. Journal of Parasitology Research, 2019. ISSN 2090-0023

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Abstract

Protein N-terminal acetylation is a co- and post-translational modification, conserved among eukaryotes. It determines the functional fate of many proteins including their stability, complex formation and subcellular localization. N-terminal acetyltransferases (NATs) transfer an acetyl group to the N-termini of proteins, and the major NATs in yeast and humans are NatA, NatB and NatC. In this study, we characterized the Trypanosoma cruzi (T. cruzi) NatC and NatA protein complexes, each consisting of one catalytic subunit and predicted auxiliary subunits. The proteins were found to be expressed in the three main life cycle stages of the parasite, formed stable complexes in vivo, and partially co-sedimented with the ribosome in agreement with a co-translational function. An in vitro acetylation assay clearly demonstrated that the acetylated substrates of the NatC catalytic subunit from T. cruzi were similar to those of yeast and human NatC, suggesting evolutionary conservation of function. An RNAi knockdown of the Trypanosome brucei (T. brucei) NatC catalytic subunit indicated that reduced NatC-mediated N-terminal acetylation of target proteins reduce parasite growth.

Item Type:	Article
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health Faculty of Medicine and Health Sciences > Research Groups > Pathogen Biology Group
Depositing User:	LivePure Connector
Date Deposited:	12 Feb 2019 09:30
Last Modified:	02 Dec 2024 01:29
URI:	https://ueaeprints.uea.ac.uk/id/eprint/69907
DOI:	10.1155/2019/6594212

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