Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure

Webber, Mark A, Whitehead, Rebekah N, Mount, Manuella, Loman, Nick J, Pallen, Mark J ORCID: https://orcid.org/0000-0003-1807-3657 and Piddock, Laura J V (2015) Parallel evolutionary pathways to antibiotic resistance selected by biocide exposure. Journal of Antimicrobial Chemotherapy, 70 (8). pp. 2241-2248. ISSN 0305-7453

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Abstract

OBJECTIVES: Biocides are widely used to prevent infection. We aimed to determine whether exposure of Salmonella to various biocides could act as a driver of antibiotic resistance. METHODS: Salmonella enterica serovar Typhimurium was exposed to four biocides with differing modes of action. Antibiotic-resistant mutants were selected during exposure to all biocides and characterized phenotypically and genotypically to identify mechanisms of resistance. RESULTS: All biocides tested selected MDR mutants with decreased antibiotic susceptibility; these occurred randomly throughout the experiments. Mutations that resulted in de-repression of the multidrug efflux pump AcrAB-TolC were seen in MDR mutants. A novel mutation in rpoA was also selected and contributed to the MDR phenotype. Other mutants were highly resistant to both quinolone antibiotics and the biocide triclosan. CONCLUSIONS: This study shows that exposure of bacteria to biocides can select for antibiotic-resistant mutants and this is mediated by clinically relevant mechanisms of resistance prevalent in human pathogens.

Item Type:	Article
Additional Information:	© The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy.
Uncontrolled Keywords:	pharmacology,drug resistance, bacterial,evolution, molecular,genotype,humans,microbial sensitivity tests,mutation,phenotype,drug effects,selection, genetic
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School Faculty of Science > School of Biological Sciences
Depositing User:	LivePure Connector
Date Deposited:	15 Jan 2019 14:30
Last Modified:	21 Oct 2022 21:33
URI:	https://ueaeprints.uea.ac.uk/id/eprint/69579
DOI:	10.1093/jac/dkv109

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