Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture

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Celiz, A D, Smith, J G W ORCID: https://orcid.org/0000-0003-0427-8678, Patel, A K, Langer, R, Anderson, D G, Barrett, D A, Young, L E, Davies, M C, Denning, C and Alexander, M R (2014) Chemically diverse polymer microarrays and high throughput surface characterisation: a method for discovery of materials for stem cell culture. Biomaterials Science, 2 (11). pp. 1604-1611. ISSN 2047-4830

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Abstract

Materials discovery provides the opportunity to identify novel materials that are tailored to complex biological environments by using combinatorial mixing of monomers to form large libraries of polymers as micro arrays. The materials discovery approach is predicated on the use of the largest chemical diversity possible, yet previous studies into human pluripotent stem cell (hPSC) response to polymer microarrays have been limited to 20 or so different monomer identities in each study. Here we show that it is possible to print and assess cell adhesion of 141 different monomers in a microarray format. This provides access to the largest chemical space to date, allowing us to meet the regenerative medicine challenge to provide scalable synthetic culture ware. This study identifies new materials suitable for hPSC expansion that could not have been predicted from previous knowledge of cell-material interactions.

Item Type: Article
Uncontrolled Keywords: sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School: Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups: Faculty of Medicine and Health Sciences > Research Groups > Cardiovascular and Metabolic Health
Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User: LivePure Connector
Date Deposited: 10 Jan 2019 16:30
Last Modified: 19 Oct 2023 02:20
URI: https://ueaeprints.uea.ac.uk/id/eprint/69547
DOI: 10.1039/c4bm00054d

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