Membrane-Spanning Electron Transfer Proteins from Electrogenic Bacteria:Production and Investigation

Lockwood, Colin, Van Wonderen, Jessica, Edwards, Marcus, Piper, Samuel, White, Gaye, Newton-Payne, Simone, Richardson, David ORCID:, Clarke, Thomas and Butt, Julea ORCID: (2018) Membrane-Spanning Electron Transfer Proteins from Electrogenic Bacteria:Production and Investigation. In: Methods in Enzymology. Elsevier, pp. 257-275.

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Certain bacterial species have a natural ability to exchange electrons with extracellular redox partners. This behavior allows coupling of catalytic transformations inside bacteria to complementary redox transformations of catalysts and electrodes outside the cell. Electricity generation can be coupled to waste-water remediation. Industrially relevant oxidation reactions proceed exclusively when electrons are released to anodes. Reduced products such as fuels can be generated when electrons are provided from (photo)cathodes. Rational development of these opportunities and inspiration for novel technologies is underpinned by resolution at the molecular level of pathways supporting electron exchange across bacterial cell envelopes. This chapter describes methods for purification, engineering and in vitro characterization of proteins providing the primary route for electron transport across the outer membrane lipid bilayer of Shewanella oneidensis MR-1, a well-described electrogenic bacterium and chassis organism for related biotechnologies.

Item Type: Book Section
Faculty \ School: Faculty of Science > School of Environmental Sciences
Faculty of Science > School of Chemistry
Faculty of Science > School of Biological Sciences
Depositing User: LivePure Connector
Date Deposited: 12 Nov 2018 14:30
Last Modified: 01 Oct 2022 00:11
DOI: 10.1016/bs.mie.2018.10.011

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