Membrane-spanning electron transfer proteins from electrogenic bacteria: Production and investigation

Lockwood, Colin W. J., van Wonderen, Jessica H., Edwards, Marcus J., Piper, Samuel E. H., White, Gaye F., Newton-Payne, Simone, Richardson, David J. ORCID: https://orcid.org/0000-0002-6847-1832, Clarke, Thomas A. ORCID: https://orcid.org/0000-0002-6234-1914 and Butt, Julea N. ORCID: https://orcid.org/0000-0002-9624-5226 (2018) Membrane-spanning electron transfer proteins from electrogenic bacteria: Production and investigation. In: Methods in Enzymology. Elsevier, pp. 257-275.

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Abstract

Certain bacterial species have a natural ability to exchange electrons with extracellular redox partners. This behavior allows coupling of catalytic transformations inside bacteria to complementary redox transformations of catalysts and electrodes outside the cell. Electricity generation can be coupled to waste-water remediation. Industrially relevant oxidation reactions proceed exclusively when electrons are released to anodes. Reduced products such as fuels can be generated when electrons are provided from (photo)cathodes. Rational development of these opportunities and inspiration for novel technologies is underpinned by resolution at the molecular level of pathways supporting electron exchange across bacterial cell envelopes. This chapter describes methods for purification, engineering and in vitro characterization of proteins providing the primary route for electron transport across the outer membrane lipid bilayer of Shewanella oneidensis MR-1, a well-described electrogenic bacterium and chassis organism for related biotechnologies.

Item Type:	Book Section
Faculty \ School:	Faculty of Science > School of Environmental Sciences Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science > School of Biological Sciences Faculty of Science > School of Natural Sciences (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Molecular Microbiology Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Organisms and the Environment Faculty of Science > Research Groups > Energy Materials Laboratory Faculty of Science > Research Groups > Chemistry of Light and Energy Faculty of Science > Research Groups > Chemistry of Life Processes
Depositing User:	LivePure Connector
Date Deposited:	12 Nov 2018 14:30
Last Modified:	24 Sep 2024 08:10
URI:	https://ueaeprints.uea.ac.uk/id/eprint/68848
DOI:	10.1016/bs.mie.2018.10.011

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