Polyamine metabolism and transforming growth factor-beta signaling are affected in Caco-2 cells by differentially cooked broccoli extracts

Furniss, Caroline S. M., Bennett, Richard N., Bacon, James R., LeGall, Gwen ORCID: https://orcid.org/0000-0002-1379-2196 and Mithen, Richard E. (2008) Polyamine metabolism and transforming growth factor-beta signaling are affected in Caco-2 cells by differentially cooked broccoli extracts. Journal of Nutrition, 138 (10). pp. 1840-1845. ISSN 0022-3166

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Abstract

The health benefits of consuming cruciferous vegetables are widely considered to be due to the biological activity of glucosinolate degradation products. However, it is conceivable that other phytochemicals within crucifers may also have biological activity that may contribute to health benefits. In this study, we analyzed global gene expression in Caco-2 cells exposed to extracts derived from broccoli that had been heat treated to different extents to result in contrasting profiles of glucosinolates and their degradation products. Extracts microwaved for 0, 1, and 4 min contained 9.5, 25.5, and 0 mu mol/L sulforaphane and induced changes in expression of 381, 1017, and 101 genes, respectively (>2 fold; P <0.01). Seventy-two genes showed similar changes in expression after treatment with all 3 extracts. These included genes involved in polyamine catabolism and transforming growth factor (TGF)-beta signaling. Consistent with these changes in gene expression, subsequent studies demonstrated that exposing cells to these extracts, including the 4-min extract that contained no glucosinolate degradation products, increased putrescine and N-acetyl-spermine concentration, and suppressed the TGF beta 1-mediated induction of phosphorylated Smad 2. This is the first report, to our knowledge, of phytochemicals from a cruciferous vegetable affecting both a signaling pathway and a catabolic process.

Item Type:	Article
Additional Information:	A correction has been published: The Journal of Nutrition, Volume 139, Issue 2, 1 February 2009, Pages 400, https://doi.org/10.3945/jn.108.103069
Uncontrolled Keywords:	human breast-cancer,phase-ii enzymes,brassica-oleracea,cycle arrest,epithiospecifier protein,dietary isothiocyanates,cruciferous vegetables,liquid-chromatography,mediated apoptosis,gene-expression,sdg 3 - good health and well-being ,/dk/atira/pure/sustainabledevelopmentgoals/good_health_and_well_being
Faculty \ School:	Faculty of Science > School of Pharmacy
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Centres > Lifespan Health
Depositing User:	LivePure Connector
Date Deposited:	26 Oct 2018 16:31
Last Modified:	19 Oct 2023 02:17
URI:	https://ueaeprints.uea.ac.uk/id/eprint/68682
DOI:	10.1093/jn/138.10.1840

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