Mass spectrometric studies of Cu(I)-binding to the N-terminal domains of B. subtilis CopA and influence of bacillithiol

Kay, Kristine L., Hamilton, Chris J. and Le Brun, Nick E. (2019) Mass spectrometric studies of Cu(I)-binding to the N-terminal domains of B. subtilis CopA and influence of bacillithiol. Journal of Inorganic Biochemistry, 190. pp. 24-30. ISSN 0162-0134

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Abstract

CopA is a Cu(I)-exporting transmembrane P1B-type ATPase from Bacillus subtilis. It contains two N-terminal cytoplasmic domains, CopAab, which bind Cu(I) with high affinity and to form higher-order complexes with multiple Cu(I) ions. To determine the precise nature of these species, electrospray ionisation mass spectrometry (ESI-MS) under non-denaturing conditions was employed. Up to 1 Cu per CopAab resulted in Cu coordination to one or both CopAab domains. At >1 Cu/CopAab, two distinct dimeric charge state envelopes were observed, corresponding to distinct conformations, each with Cu6(CopAab)2 as its major form. The influence of the physiologically relevant low molecular weight thiol bacillithiol (BSH) on Cu(I)-binding to CopAab was assessed. Dimeric CopAab persisted in the presence of BSH, with previously undetected Cu7(CopAab)2 and Cu6(CopAab)2(BSH) forms apparent.

Item Type:	Article
Uncontrolled Keywords:	copper trafficking,electrospray ionisation mass spectrometry,bacillus subtilis,copper-mediated association,bacillithiol,biochemistry,inorganic chemistry ,/dk/atira/pure/subjectarea/asjc/1300/1303
Faculty \ School:	Faculty of Science > School of Chemistry (former - to 2024) Faculty of Science > School of Pharmacy (former - to 2024)
UEA Research Groups:	Faculty of Science > Research Groups > Chemical Biology and Medicinal Chemistry (former - to 2021) Faculty of Science > Research Centres > Centre for Molecular and Structural Biochemistry Faculty of Science > Research Groups > Chemistry of Life Processes (former - to 2025) Faculty of Science > Research Groups > Pharmacology and Biological Chemistry Faculty of Science > Research Groups > Synthetic and Medicinal Chemistry
Related URLs:	http://www.scopus.com/inward/record.url?... https://linkinghub.elsevier.com/retrieve...
Depositing User:	LivePure Connector
Date Deposited:	16 Oct 2018 09:30
Last Modified:	06 Feb 2026 09:31
URI:	https://ueaeprints.uea.ac.uk/id/eprint/68541
DOI:	10.1016/j.jinorgbio.2018.10.004

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