Tools
ToolsBrulé, Daphnée, Villano, Clizia, Davies, Laura J., Trdá, Lucie, Claverie, Justine, Héloir, Marie-Claire, Chiltz, Annick, Adrian, Marielle, Darblade, Benoît, Tornero, Pablo, Stransfeld, Lena, Boutrot, Freddy, Zipfel, Cyril, Dry, Ian B. and Poinssot, Benoit (2019) The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnology Journal, 17 (4). pp. 812-825. ISSN 1467-7644
Preview |
PDF (Accepted manuscript)
- Accepted Version
Available under License Creative Commons Attribution. Download (1MB) | Preview |
Abstract
Chitin, a major component of fungal cell walls, is a well-known pathogen-associated molecular pattern (PAMP) that triggers defense responses in several mammal and plant species. Here, we show that two chitooligosaccharides, chitin and chitosan, act as PAMPs in grapevine (Vitis vinifera) as they elicit immune signalling events, defense gene expression and resistance against fungal diseases. To identify their cognate receptors, the grapevine family of LysM receptor kinases (LysM-RKs) was annotated and their gene expression profiles were characterized. Phylogenetic analysis clearly distinguished three V. vinifera LysM-RKs (VvLYKs) located in the same clade as the Arabidopsis CHITIN ELICITOR RECEPTOR KINASE1 (AtCERK1), which mediates chitin-induced immune responses. The Arabidopsis mutant Atcerk1, impaired in chitin perception, was transformed with these three putative orthologous genes encoding VvLYK1-1, -2, or -3 to determine if they would complement the loss of AtCERK1 function. Our results provide evidence that VvLYK1-1 and VvLYK1-2, but not VvLYK1-3, functionally complement the Atcerk1 mutant by restoring chitooligosaccharide-induced MAPK activation and immune gene expression. Moreover, expression of VvLYK1-1 in Atcerk1 restored penetration resistance to the non-adapted grapevine powdery mildew (Erysiphe necator). On the whole, our results indicate that the grapevine VvLYK1-1 and VvLYK1-2 participate in chitin- and chitosan-triggered immunity and that VvLYK1-1 plays an important role in basal resistance against E. necator.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | erysiphe necator,vitis vinifera,immune responses,pathogen-associated molecular pattern,pattern recognition receptor,resistance,biotechnology,agronomy and crop science,plant science ,/dk/atira/pure/subjectarea/asjc/1300/1305 |
| Faculty \ School: | Faculty of Science > The Sainsbury Laboratory |
| UEA Research Groups: | Faculty of Science > Research Groups > Plant Sciences |
| Related URLs: | |
| Depositing User: | LivePure Connector |
| Date Deposited: | 12 Oct 2018 11:30 |
| Last Modified: | 21 May 2024 13:30 |
| URI: | https://ueaeprints.uea.ac.uk/id/eprint/68503 |
| DOI: | 10.1111/pbi.13017 |
Downloads
Downloads per month over past year
Actions (login required)
 |
View Item |