STD NMR as a Technique for Ligand Screening and Structural Studies

Walpole, Samuel, Monaco, Serena, Nepravishta, Ridvan and Angulo, Jesus ORCID: https://orcid.org/0000-0001-7250-5639 (2019) STD NMR as a Technique for Ligand Screening and Structural Studies. In: Methods in Enzymology. Elsevier, pp. 423-451.

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Abstract

STD NMR is a powerful ligand-based tool for screening small molecules and low molecular weight fragments for their interaction with a given macromolecule. Such information is invaluable both in the drug discovery sector and in understanding fundamental biological interactions. Recently, powerful methods have been developed to extract a greater wealth of information from the STD NMR experiment, including ligand binding epitopes, dissociation constant determination, and mapping of binding site properties. Herein we describe these STD NMR experiments, giving practical examples for each approach, and highlight the important parameters and common pitfalls that must be considered for a successful experiment.

Item Type:	Book Section
Uncontrolled Keywords:	saturation transfer difference nmr,std nmr,protein–ligand interactions,ligand screening,deep-std nmr,binding epitope mapping
Faculty \ School:	Faculty of Science > School of Pharmacy
UEA Research Groups:	Faculty of Science > Research Groups > Pharmaceutical Materials and Soft Matter
Depositing User:	LivePure Connector
Date Deposited:	20 Sep 2018 16:30
Last Modified:	21 Oct 2022 19:30
URI:	https://ueaeprints.uea.ac.uk/id/eprint/68320
DOI:	10.1016/bs.mie.2018.08.018

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