Comparative proteomics provides insights into metabolic responses in rat liver to isolated soy and meat proteins

Song, Shangxin, Hooiveld, Guido J., Zhang, Wei, Li, Mengjie, Zhao, Fan, Zhu, Jing, Xu, Xinglian, Muller, Michael ORCID: https://orcid.org/0000-0002-5930-9905, Li, Chunbao and Zhou, Guanghong (2016) Comparative proteomics provides insights into metabolic responses in rat liver to isolated soy and meat proteins. Journal of Proteome Research, 15 (4). 1135–1142. ISSN 1535-3893

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Abstract

It has been reported that isolated dietary soy and meat proteins have distinct effects on physiology and liver gene expression, but the impact on protein expression responses are unknown. Because these may differ from gene expression responses, we investigated dietary protein-induced changes in liver proteome. Rats were fed for 1 week semisynthetic diets that differed only regarding protein source; casein (reference) was fully replaced by isolated soy, chicken, fish, or pork protein. Changes in liver proteome were measured by iTRAQ labeling and LC–ESI–MS/MS. A robust set totaling 1437 unique proteins was identified and subjected to differential protein analysis and biological interpretation. Compared with casein, all other protein sources reduced the abundance of proteins involved in fatty acid metabolism and Pparα signaling pathway. All dietary proteins, except chicken, increased oxidoreductive transformation reactions but reduced energy and essential amino acid metabolic pathways. Only soy protein increased the metabolism of sulfur-containing and nonessential amino acids. Soy and fish proteins increased translation and mRNA processing, whereas only chicken protein increased TCA cycle but reduced immune responses. These findings were partially in line with previously reported transcriptome results. This study further shows the distinct effects of soy and meat proteins on liver metabolism in rats.

Item Type:	Article
Uncontrolled Keywords:	metabolic syndrome,isolated protein,animal protein,chicken protein,fish protein,pork protein,molecular nutrition,nutrigenomics,proteomics
Faculty \ School:	Faculty of Medicine and Health Sciences > Norwich Medical School
UEA Research Groups:	Faculty of Medicine and Health Sciences > Research Groups > Gastroenterology and Gut Biology Faculty of Medicine and Health Sciences > Research Groups > Nutrition and Preventive Medicine Faculty of Medicine and Health Sciences > Research Centres > Metabolic Health
Depositing User:	LivePure Connector
Date Deposited:	02 Aug 2018 11:30
Last Modified:	06 Jun 2024 15:03
URI:	https://ueaeprints.uea.ac.uk/id/eprint/67949
DOI:	10.1021/acs.jproteome.5b00922

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